Wee1 Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: EC; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Protein kinase, tyrosine (non-receptor); WEE family
Chromosomal Location of Human Ortholog: 7|7 E3
Cellular Component:  cytoplasm; nucleolus; nucleus
Molecular Function:  ATP binding; kinase activity; magnesium ion binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein kinase activity; protein tyrosine kinase activity; transferase activity
Biological Process:  cell cycle; cell division; establishment of cell polarity; microtubule cytoskeleton organization; mitotic cell cycle; mitotic cell cycle checkpoint; neuron projection morphogenesis; peptidyl-tyrosine phosphorylation; phosphorylation; protein phosphorylation
Reference #:  P47810 (UniProtKB)
Alt. Names/Synonyms: WEE 1 homolog 1; WEE 1 homolog 1 (S. pombe); Wee1; WEE1 tyrosine kinase; Wee1-like protein kinase; Wee1A; Wee1A kinase
Gene Symbols: Wee1
Molecular weight: 71,578 Da
Basal Isoelectric point: 5.85  Predict pI for various phosphorylation states
CST Pathways:  G2/M DNA Damage Checkpoint  |  PI3K/Akt Signaling
Select Structure to View Below


Protein Structure Not Found.

Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  KinBase  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein