DAPK3 Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. Widely expressed. Isoform 1 and isoform 2 are expressed in the bladder smooth muscle. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: CAMK group; DAPK family; EC; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor
Chromosomal Location of human Ortholog: 19p13.3
Cellular Component:  actin filament; membrane raft; nucleus; PML body
Molecular Function:  ATP binding; cAMP response element binding protein binding; identical protein binding; leucine zipper domain binding; protein binding; protein C-terminus binding; protein homodimerization activity; protein serine/threonine kinase activity; Rho GTPase binding
Biological Process:  apoptotic process; apoptotic signaling pathway; cellular response to interferon-gamma; chromatin organization; intracellular signal transduction; negative regulation of translation; neuron differentiation; positive regulation of apoptotic process; positive regulation of canonical Wnt signaling pathway; positive regulation of cell migration; positive regulation of extrinsic apoptotic signaling pathway in absence of ligand; protein autophosphorylation; protein phosphorylation; regulation of actin cytoskeleton reorganization; regulation of apoptotic process; regulation of autophagy; regulation of cell motility; regulation of cell shape; regulation of focal adhesion assembly; regulation of mitotic cell cycle; regulation of mitotic nuclear division; regulation of myosin II filament organization; regulation of smooth muscle contraction; regulation of transcription, DNA-templated
Reference #:  O43293 (UniProtKB)
Alt. Names/Synonyms: DAP kinase 3; DAP-like kinase; DAPK3; death associated protein kinase 3; Death-associated protein kinase 3; Dlk; FLJ36473; MYPT1 kinase; ZIP; ZIP kinase isoform; ZIP-kinase; ZIPK; Zipper-interacting protein kinase
Gene Symbols: DAPK3
Molecular weight: 52,536 Da
Basal Isoelectric point: 6.44  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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