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DGCR8
Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing. Involved in the silencing of embryonic stem cell self-renewal. Ubiquitously expressed. 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
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| Protein type: Nucleolus; RNA processing; RNA-binding |
| Chromosomal Location of human Ortholog: 22q11.21 |
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Cellular Component:
cytoplasm; cytosol; glutamatergic synapse; microprocessor complex; nuclear body; nucleolus; nucleoplasm; nucleus; postsynaptic density
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Molecular Function:
double-stranded RNA binding; heme binding; identical protein binding; metal ion binding; primary miRNA binding; protein binding; protein homodimerization activity; protein-RNA adaptor activity
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Biological Process:
positive regulation of pre-miRNA processing; primary miRNA processing; regulation of stem cell proliferation
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Disease: Velocardiofacial Syndrome
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Reference #:
Q8WYQ5
(UniProtKB)
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Alt. Names/Synonyms: C22orf12; DGCR8; DGCR8 microprocessor complex subunit; DGCRK6; DiGeorge syndrome critical region 8; DiGeorge syndrome critical region gene 8; Gy1; Microprocessor complex subunit DGCR8; pasha
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Gene Symbols: DGCR8
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Molecular weight:
86,045 Da
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Basal Isoelectric point:
5.68
Predict pI for various phosphorylation states
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Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology®
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