IRF3 Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages. Belongs to the IRF family. Expressed constitutively in a variety of tissues. 5 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of human Ortholog: 19q13.33
Cellular Component:  cytoplasm; cytosol; nucleoplasm; nucleus
Molecular Function:  DNA binding; DNA-binding transcription activator activity, RNA polymerase II-specific; DNA-binding transcription factor activity, RNA polymerase II-specific; identical protein binding; protein binding; protein domain specific binding; protein homodimerization activity; RNA polymerase II proximal promoter sequence-specific DNA binding; sequence-specific DNA binding; transcription coregulator activity
Biological Process:  apoptotic process; cellular response to DNA damage stimulus; cellular response to exogenous dsRNA; defense response to virus; interferon-gamma-mediated signaling pathway; macrophage apoptotic process; MDA-5 signaling pathway; negative regulation of type I interferon production; positive regulation of cytokine secretion; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; positive regulation of transcription by RNA polymerase II; positive regulation of type I interferon production; regulation of type I interferon production; transcription by RNA polymerase II; TRIF-dependent toll-like receptor signaling pathway; type I interferon signaling pathway; viral process
Disease: Herpes Simplex Encephalitis, Susceptibility To, 7
Reference #:  Q14653 (UniProtKB)
Alt. Names/Synonyms: IIAE7; Interferon regulatory factor 3; IRF-3; IRF3
Gene Symbols: IRF3
Molecular weight: 47,219 Da
Basal Isoelectric point: 5.17  Predict pI for various phosphorylation states
CST Pathways:  Toll-Like Receptor Signaling
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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IRF3

Protein Structure Not Found.


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