NBS1 Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex. Ubiquitous. Expressed at high levels in testis. Note: This description may include information from UniProtKB.
Protein type: Cell cycle regulation; DNA repair, damage
Chromosomal Location of mouse Ortholog: 4|4 A2
Cellular Component:  chromosome; chromosome, telomeric region; Mre11 complex; nuclear chromosome, telomeric region; nuclear inclusion body; nucleolus; nucleus; PML body; replication fork; site of double-strand break
Molecular Function:  damaged DNA binding; protein binding; protein N-terminus binding; transcription factor binding
Biological Process:  blastocyst growth; cell cycle; cell proliferation; cellular response to DNA damage stimulus; DNA damage checkpoint; DNA duplex unwinding; DNA repair; double-strand break repair; double-strand break repair via homologous recombination; in utero embryonic development; intrinsic apoptotic signaling pathway; isotype switching; meiotic cell cycle; mitotic cell cycle checkpoint; mitotic G2 DNA damage checkpoint; negative regulation of neuron differentiation; negative regulation of telomere capping; negative regulation of viral entry into host cell; neuromuscular process controlling balance; positive regulation of cell proliferation; positive regulation of kinase activity; positive regulation of protein autophosphorylation; positive regulation of telomere maintenance; regulation of fibroblast proliferation; signal transduction in response to DNA damage; t-circle formation; telomere capping; telomere maintenance; telomere maintenance via telomere trimming; telomeric 3' overhang formation
Reference #:  Q9R207 (UniProtKB)
Alt. Names/Synonyms: Cell cycle regulatory protein p95; Nbn; Nbs1; Nibrin; Nijmegen breakage syndrome protein 1 homolog; OTTMUSP00000004959
Gene Symbols: Nbn
Molecular weight: 83,795 Da
Basal Isoelectric point: 6.28  Predict pI for various phosphorylation states
CST Pathways:  G2/M DNA Damage Checkpoint  |  Protein Acetylation
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  Reactome  |  BioGPS  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene