PKCE Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardiomyocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. In HeLa cells, contributes to hepatocyte growth factor (HGF)-induced cell migration, and in human corneal epithelial cells, plays a critical role in wound healing after activation by HGF. During cytokinesis, forms a complex with YWHAB, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implicate the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaction with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a role in the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). Involved in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be involved in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin- and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. Is able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). In prostate cancer cells, interacts with and phosphorylates STAT3, which increases DNA-binding and transcriptional activity of STAT3 and seems to be essential for prostate cancer cell invasion. Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines production. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Note: This description may include information from UniProtKB.
Protein type: AGC group; EC 2.7.11.13; Eta subfamily; Kinase, protein; PKC family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 17|17 E4
Cellular Component:  cell periphery; cytoplasm; cytoskeleton; cytosol; endoplasmic reticulum; glutamatergic synapse; Golgi membrane; membrane; mitochondrion; neuromuscular junction; nucleus; perinuclear region of cytoplasm; plasma membrane; presynaptic cytosol; T-tubule
Molecular Function:  14-3-3 protein binding; actin monomer binding; ATP binding; calcium-independent protein kinase C activity; enzyme activator activity; enzyme binding; ethanol binding; kinase activity; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein kinase binding; protein kinase C activity; protein serine/threonine kinase activity; receptor activator activity; SH3 domain binding; signaling receptor binding; transferase activity
Biological Process:  cell adhesion; cell cycle; cell division; cellular response to ethanol; cellular response to hypoxia; cellular response to platelet-derived growth factor stimulus; cellular response to prostaglandin E stimulus; chemosensory behavior; immune system process; intracellular signal transduction; lipopolysaccharide-mediated signaling pathway; locomotory exploration behavior; macrophage activation involved in immune response; negative regulation of apoptotic process; negative regulation of mitochondrial calcium ion concentration; negative regulation of mitochondrial membrane potential; negative regulation of protein ubiquitination; negative regulation of release of sequestered calcium ion into cytosol; peptidyl-serine phosphorylation; phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell-substrate adhesion; positive regulation of cellular glucuronidation; positive regulation of cytokinesis; positive regulation of epithelial cell migration; positive regulation of fibroblast migration; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of insulin secretion; positive regulation of lipid catabolic process; positive regulation of MAPK cascade; positive regulation of mucus secretion; positive regulation of synaptic transmission, GABAergic; positive regulation of wound healing; protein phosphorylation; regulation of insulin secretion involved in cellular response to glucose stimulus; regulation of lipid metabolic process; regulation of peptidyl-tyrosine phosphorylation; regulation of release of sequestered calcium ion into cytosol; regulation of synaptic vesicle exocytosis; response to morphine; TRAM-dependent toll-like receptor 4 signaling pathway
Reference #:  P16054 (UniProtKB)
Alt. Names/Synonyms: 5830406C15Rik; KPCE; nPKC-epsilon; OTTMUSP00000019117; OTTMUSP00000019118; Pkc; PKC[e; PKC[e]; Pkce; Pkcea; PKCepsilon; Prkce; Protein kinase C epsilon type; protein kinase C, epsilon; R75156
Gene Symbols: Prkce
Molecular weight: 83,561 Da
Basal Isoelectric point: 6.62  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  B Cell Receptor Signaling  |  ErbB/HER Signaling  |  GPCR Signaling to MAPKs  |  Growth And Differentiation Control by MAPKs  |  Inhibition of Apoptosis  |  Mitochondrial Control of Apoptosis  |  Phospholipase Signaling
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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PKCE

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Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  KinBase  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene