DAPK1 Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition. Isoform 2 cannot induce apoptosis but can induce membrane blebbing. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas. 4 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Apoptosis; Autophagy; CAMK group; DAPK family; EC 2.7.11.1; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 13 B2|13 32.53 cM
Cellular Component:  actin cytoskeleton; cytoplasm; nucleus; plasma membrane
Molecular Function:  ATP binding; calmodulin binding; calmodulin-dependent protein kinase activity; GTP binding; identical protein binding; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; syntaxin-1 binding; transferase activity
Biological Process:  apoptotic process; apoptotic signaling pathway; cellular response to hydroperoxide; cellular response to interferon-gamma; extrinsic apoptotic signaling pathway via death domain receptors; intracellular signal transduction; negative regulation of apoptotic process; negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; negative regulation of translation; phosphorylation; positive regulation of autophagy; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; protein autophosphorylation; protein phosphorylation; regulation of NMDA receptor activity; regulation of translation; signal transduction
Reference #:  Q80YE7 (UniProtKB)
Alt. Names/Synonyms: 2310039H24Rik; 2810425C21Rik; AI642003; D13Ucla1; DAP kinase 1; DAP-Kinase; Dapk1; death associated protein kinase 1; Death-associated protein kinase 1
Gene Symbols: Dapk1
Molecular weight: 161,442 Da
Basal Isoelectric point: 6.43  Predict pI for various phosphorylation states
CST Pathways:  Death Receptor Signaling  |  Growth And Differentiation Control by MAPKs
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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DAPK1

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Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  KinBase  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene