CtIP Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations. Belongs to the COM1/SAE2/CtIP family. Expressed in ER-positive breast cancer lines, but tends to be down-regulated ER-negative cells (at protein level). 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: EC 3.1.-.-; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 18|18 A1
Cellular Component:  chromosome; intracellular membrane-bounded organelle; nucleoplasm; nucleus; site of double-strand break; transcriptional repressor complex
Molecular Function:  damaged DNA binding; DNA binding; double-strand/single-strand DNA junction binding; double-stranded DNA binding; endonuclease activity; flap-structured DNA binding; hydrolase activity; identical protein binding; nuclease activity; RNA polymerase II repressing transcription factor binding; single-stranded DNA binding; single-stranded DNA endodeoxyribonuclease activity; transcription corepressor activity; Y-form DNA binding
Biological Process:  blastocyst hatching; cell cycle; cell division; cellular response to DNA damage stimulus; DNA double-strand break processing involved in repair via single-strand annealing; DNA repair; double-strand break repair via homologous recombination; G1/S transition of mitotic cell cycle; meiotic cell cycle; nucleotide-excision repair
Reference #:  Q80YR6 (UniProtKB)
Alt. Names/Synonyms: 9930104E21Rik; CtIP; DNA endonuclease RBBP8; Rbbp8; Rbbp8 protein; retinoblastoma binding protein 8
Gene Symbols: Rbbp8
Molecular weight: 100,831 Da
Basal Isoelectric point: 6.26  Predict pI for various phosphorylation states
Select Structure to View Below


Protein Structure Not Found.

Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein