POLD1 As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites. Belongs to the DNA polymerase type-B family. Widely expressed, with high levels of expression in heart and lung. Note: This description may include information from UniProtKB.
Protein type: DNA repair, damage; DNA replication; EC 2.7.7.7; Hydrolase; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transferase
Chromosomal Location of mouse Ortholog: 7 B3|7 28.83 cM
Cellular Component:  aggresome; cytosol; delta DNA polymerase complex; nucleoplasm; nucleotide-excision repair complex; nucleus
Molecular Function:  3'-5' exonuclease activity; 3'-5'-exodeoxyribonuclease activity; 4 iron, 4 sulfur cluster binding; chromatin binding; damaged DNA binding; DNA binding; DNA-directed DNA polymerase activity; enzyme binding; exonuclease activity; hydrolase activity; iron-sulfur cluster binding; metal ion binding; nuclease activity; nucleic acid binding; nucleotide binding; nucleotidyltransferase activity; transferase activity
Biological Process:  base-excision repair, gap-filling; cellular response to DNA damage stimulus; cellular response to UV; DNA biosynthetic process; DNA repair; DNA replication; DNA replication proofreading; DNA synthesis involved in DNA repair; DNA-dependent DNA replication; fatty acid homeostasis; nucleotide-excision repair, DNA gap filling
Reference #:  P52431 (UniProtKB)
Alt. Names/Synonyms: 125kD; 125kDa; 3'-5' exodeoxyribonuclease; DNA polymerase delta 1, catalytic domain; DNA polymerase delta catalytic subunit; DPOD1; Pold1; polymerase (DNA directed), delta 1, catalytic subunit
Gene Symbols: Pold1
Molecular weight: 123,790 Da
Basal Isoelectric point: 7.69  Predict pI for various phosphorylation states
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POLD1

Protein Structure Not Found.


Cross-references to other databases:  AlphaFold  |  STRING  |  Reactome  |  BioGPS  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene