UHRF1 Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD- type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair. Defects in UHRF1 may be a cause of cancers. Overexpressed in many different forms of human cancers, including bladder, breast, cervical, colorectal and prostate cancers, as well as pancreatic adenocarcinomas, rhabdomyosarcomas and gliomas. Plays an important role in the correlation of histone modification and gene silencing in cancer progression. Expression is associated with a poor prognosis in patients with various cancers, suggesting that it participates in cancer progression. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA-binding; EC 6.3.2.-; EC 6.3.2.19; Ligase; Transcription factor; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 17|17 D
Cellular Component:  cytoplasmic vesicle; euchromatin; heterochromatin; nuclear chromatin; nuclear heterochromatin; nuclear matrix; nucleus; plasma membrane; replication fork
Molecular Function:  DNA binding; histone binding; identical protein binding; metal ion binding; methyl-CpG binding; methylated histone residue binding; nucleosomal histone binding; protein binding; transferase activity; ubiquitin-protein transferase activity; zinc ion binding
Biological Process:  cell cycle; cell proliferation; cellular response to DNA damage stimulus; covalent chromatin modification; DNA repair; histone monoubiquitination; histone ubiquitination; maintenance of DNA methylation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cellular protein metabolic process; protein autoubiquitination; protein ubiquitination involved in ubiquitin-dependent protein catabolic process; regulation of transcription, DNA-templated; transcription, DNA-dependent
Reference #:  Q8VDF2 (UniProtKB)
Alt. Names/Synonyms: AL022808; E3 ubiquitin-protein ligase UHRF1; ICBP90; Np95; Nuclear protein 95; Nuclear zinc finger protein Np95; OTTMUSP00000023097; OTTMUSP00000023100; RNF106; Ubiquitin-like PHD and RING finger domain-containing protein 1; ubiquitin-like, containing PHD and RING finger domains, 1; Ubiquitin-like-containing PHD and RING finger domains protein 1; Uhrf1
Gene Symbols: Uhrf1
Molecular weight: 88,304 Da
Basal Isoelectric point: 8.63  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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UHRF1

Protein Structure Not Found.


Cross-references to other databases:  STRING  |  BioGPS  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene