ALKBH5 Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells. Belongs to the alkB family. Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary. 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: DNA repair, damage; EC 1.14.11.-; Membrane protein, integral; Oxidoreductase; RNA-binding
Chromosomal Location of Human Ortholog: 17p11.2|17p11.2
Cellular Component:  cytosol; Golgi apparatus; nuclear speck; nucleoplasm; nucleus
Molecular Function:  metal ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; RNA N6-methyladenosine dioxygenase activity
Biological Process:  cell differentiation; DNA dealkylation involved in DNA repair; mRNA export from nucleus; mRNA processing; oxidative single-stranded RNA demethylation; regulation of mRNA stability; response to hypoxia; spermatogenesis
Reference #:  Q6P6C2 (UniProtKB)
Alt. Names/Synonyms: ABH5; AlkB family member 5, RNA demethylase; alkB homolog 5, RNA demethylase; alkB, alkylation repair homolog 5; alkB, alkylation repair homolog 5 (E. coli); ALKB5; ALKBH5; Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5; FLJ20308; OFOXD; OFOXD1; oxoglutarate and iron-dependent oxygenase domain containing; probable alpha-ketoglutarate-dependent dioxygenase ABH5; RNA demethylase ALKBH5
Gene Symbols: ALKBH5
Molecular weight: 44,256 Da
Basal Isoelectric point: 9.19  Predict pI for various phosphorylation states
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Protein Structure Not Found.

Cross-references to other databases:  STRING  |  cBioPortal  |  Wikipedia  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene