CPSF3 Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily. Note: This description may include information from UniProtKB.
Protein type: EC 3.1.27.-; RNA processing; RNA splicing; RNA-binding; Ribonuclease
Chromosomal Location of human Ortholog: 2p25.1
Cellular Component:  mRNA cleavage and polyadenylation specificity factor complex; nucleoplasm; ribonucleoprotein complex
Molecular Function:  5'-3' exonuclease activity; 5'-3' exoribonuclease activity; endoribonuclease activity; metal ion binding; protein binding; RNA binding
Biological Process:  mRNA 3'-end processing; mRNA 3'-end processing by stem-loop binding and cleavage; mRNA cleavage; mRNA polyadenylation; positive regulation of G1/S transition of mitotic cell cycle; RNA phosphodiester bond hydrolysis, endonucleolytic; RNA phosphodiester bond hydrolysis, exonucleolytic
Disease: Neurodevelopmental Disorder With Microcephaly, Hypotonia, And Seizures
Reference #:  Q9UKF6 (UniProtKB)
Alt. Names/Synonyms: cleavage and polyadenylation specific factor 3; cleavage and polyadenylation specific factor 3, 73kDa; cleavage and polyadenylation specificity factor 3; Cleavage and polyadenylation specificity factor 73 kDa subunit; Cleavage and polyadenylation specificity factor subunit 3; CPSF; CPSF 73 kDa subunit; CPSF-73; CPSF3; CPSF73; mRNA 3'-end-processing endonuclease CPSF-73; YSH1
Gene Symbols: CPSF3
Molecular weight: 77,486 Da
Basal Isoelectric point: 5.37  Predict pI for various phosphorylation states
Select Structure to View Below


Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  cBioPortal  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene