KLHL22
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation. The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway. It is therefore an amino acid-dependent activator within the amino acid-sensing branch of the TORC1 pathway, indirectly regulating different cellular processes including cell growth and autophagy. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
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Protein type: Ubiquitin conjugating system |
Chromosomal Location of human Ortholog: 22q11.21 |
Cellular Component:
centrosome; Cul3-RING ubiquitin ligase complex; cytoplasm; cytosol; intercellular bridge; intracellular membrane-bounded organelle; lysosome; microtubule cytoskeleton; mitotic spindle; nucleus; polar microtubule
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Molecular Function:
14-3-3 protein binding; protein binding; ubiquitin ligase-substrate adaptor activity
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Biological Process:
cell division; cellular response to leucine; mitotic sister chromatid segregation; mitotic spindle assembly checkpoint signaling; negative regulation of autophagy; negative regulation of type I interferon production; positive regulation of cell growth; positive regulation of TORC1 signaling; proteasome-mediated ubiquitin-dependent protein catabolic process; protein monoubiquitination; ubiquitin-dependent protein catabolic process
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Reference #:
Q53GT1
(UniProtKB)
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Alt. Names/Synonyms: kelch like family member 22; kelch-like 22; kelch-like 22 (Drosophila); Kelch-like protein 22; KELCHL; KLH22; KLHL22
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Gene Symbols: KLHL22
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Molecular weight:
71,667 Da
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Basal Isoelectric point:
5.28
Predict pI for various phosphorylation states
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