HSF1 a transcription factor that specifically binds heat shock promoter elements (HSE), inducing expression of the heat shock response (HSR), a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage. Induced in response to heat, heavy metals, and oxidative stress. In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form. Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes. Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form. Binds to inverted 5'-NGAAN-3' pentamer DNA sequences. Binds to chromatin at heat shock gene promoters. Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells. Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner. Plays a role in nuclear export of stress-induced HSP70 mRNA. Plays a role in the regulation of mitotic progression. Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner. Involved in stress-induced cancer cell proliferation in a IER5-dependent manner. Belongs to the HSF family. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of human Ortholog: 8q24.3
Cellular Component:  centrosome; chromatin; cytoplasm; cytosol; euchromatin; heterochromatin; kinetochore; mitotic spindle pole; nuclear stress granule; nucleoplasm; nucleus; perinuclear region of cytoplasm; PML body; protein folding chaperone complex; ribonucleoprotein complex
Molecular Function:  chromatin DNA binding; DNA binding; DNA-binding transcription activator activity, RNA polymerase II-specific; DNA-binding transcription factor activity; DNA-binding transcription factor activity, RNA polymerase II-specific; DNA-binding transcription repressor activity, RNA polymerase II-specific; general transcription initiation factor binding; heat shock protein binding; Hsp90 protein binding; identical protein binding; promoter-specific chromatin binding; protein binding; protein heterodimerization activity; protein kinase binding; protein self-association; RNA polymerase II cis-regulatory region sequence-specific DNA binding; RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding; sequence-specific DNA binding; sequence-specific double-stranded DNA binding; sequence-specific single stranded DNA binding; STAT family protein binding; transcription cis-regulatory region binding; translation elongation factor binding
Biological Process:  cellular response to angiotensin; cellular response to cadmium ion; cellular response to copper ion; cellular response to diamide; cellular response to estradiol stimulus; cellular response to gamma radiation; cellular response to heat; cellular response to hydrogen peroxide; cellular response to L-glutamine; cellular response to lipopolysaccharide; cellular response to nitroglycerin; cellular response to potassium ion; cellular response to sodium arsenite; cellular response to unfolded protein; cellular response to xenobiotic stimulus; defense response; DNA repair; MAPK cascade; mRNA processing; mRNA transport; negative regulation of cardiac muscle cell apoptotic process; negative regulation of double-strand break repair via nonhomologous end joining; negative regulation of gene expression; negative regulation of inclusion body assembly; negative regulation of neuron death; negative regulation of protein-containing complex assembly; negative regulation of transcription by RNA polymerase II; positive regulation of apoptotic DNA fragmentation; positive regulation of cold-induced thermogenesis; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of DNA-binding transcription factor activity; positive regulation of gene expression; positive regulation of inclusion body assembly; positive regulation of macrophage differentiation; positive regulation of microtubule binding; positive regulation of mitotic cell cycle; positive regulation of transcription by RNA polymerase II; positive regulation of tyrosine phosphorylation of STAT protein; protein-containing complex assembly; regulation of cellular response to heat; regulation of transcription by RNA polymerase II; response to activity; response to hypobaric hypoxia; response to nutrient; response to psychosocial stress; response to testosterone
Reference #:  Q00613 (UniProtKB)
Alt. Names/Synonyms: Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; HSF1; HSTF 1; HSTF1
Gene Symbols: HSF1
Molecular weight: 57,260 Da
Basal Isoelectric point: 5.02  Predict pI for various phosphorylation states
CST Pathways:  SAPK/JNK Signaling Cascades
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
Select Structure to View Below


Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  cBioPortal  |  Wikipedia  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein