WNK3
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner. Isoform 1, isoform 2, isoform 3 and isoform 4 stimulate the activity of SLC12A1, SLC12A2 and SLC12A3 and inhibit the activity of SLC12A4, SLC12A5, SLC12A6 and SLC12A7. According to PubMed:19470686, isoform 1 inhibits the activity of SLC12A3. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. Expressed in brain, lung, kidney, liver and pancreas, and in fetal tissues including placenta, fetal brain, lung and kidney. Very low levels of expression were also detected in fetal heart, thymus, liver and spleen. Isoform 1 is brain-specific. Isoform 3 is kidney-specific. 4 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.1; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Wnk family
Molecular Function: ATP binding; kinase activity; molecular condensate scaffold activity; nucleotide binding; potassium channel inhibitor activity; protein kinase activity; protein serine/threonine kinase activity; transferase activity
Biological Process: cell volume homeostasis; cellular hyperosmotic response; intracellular signal transduction; maintenance of blood-brain barrier; monoatomic ion homeostasis; negative regulation of apoptotic process; negative regulation of pancreatic juice secretion; negative regulation of protein localization to plasma membrane; negative regulation of sodium ion transport; non-membrane-bounded organelle assembly; osmosensory signaling pathway; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of calcium ion transport; positive regulation of ion transmembrane transporter activity; positive regulation of peptidyl-threonine phosphorylation; positive regulation of potassium ion import across plasma membrane; positive regulation of protein localization to plasma membrane; positive regulation of sodium ion transmembrane transporter activity; positive regulation of sodium ion transport; protein autophosphorylation; protein localization to plasma membrane; protein phosphorylation; regulation of calcium ion import; regulation of monoatomic cation transmembrane transport
