AAK1 Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: BIKE subfamily; EC; Kinase, protein; NAK family; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of mouse Ortholog: 6 D1|6 37.75 cM
Cellular Component:  calyx of Held; cell leading edge; cell projection; clathrin complex; clathrin-coated pit; membrane; plasma membrane; presynapse; synapse; terminal bouton
Molecular Function:  AP-2 adaptor complex binding; ATP binding; kinase activity; Notch binding; nucleotide binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity
Biological Process:  endocytosis; phosphorylation; positive regulation of Notch signaling pathway; presynaptic endocytosis; protein autophosphorylation; protein phosphorylation; protein stabilization; regulation of clathrin-dependent endocytosis; regulation of protein localization
Reference #:  Q3UHJ0 (UniProtKB)
Alt. Names/Synonyms: 5530400K14Rik; 9630042K20; Aak1; Adaptor-associated kinase 1; AP2 associated kinase 1; AP2-associated protein kinase 1; AU067724; AU067726; BC028270; C79663; D6Ertd245; D6Ertd245e; Gm38495; Kiaa1048; MGC40785; mKIAA1048; OTTMUSP00000029283; OTTMUSP00000029285; R75501
Gene Symbols: Aak1
Molecular weight: 103,346 Da
Basal Isoelectric point: 6.25  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  BioGPS  |  KinBase  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene