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POLR3A
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the RNA polymerase beta' chain family. Expressed in the brain, in the cortex and the white matter (at protein level). Note: This description may include information from UniProtKB.
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| Protein type: EC 2.7.7.6; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transcription initiation complex; Transferase |
| Chromosomal Location of mouse Ortholog: 14|14 A3 |
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Cellular Component:
nucleoplasm; nucleus; RNA polymerase III complex
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Molecular Function:
chromatin binding; DNA-directed 5'-3' RNA polymerase activity; RNA polymerase III activity
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Biological Process:
innate immune response; positive regulation of interferon-beta production
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Reference #:
B2RXC6
(UniProtKB)
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Alt. Names/Synonyms: 9330175N20Rik; BC053071; DNA-directed RNA polymerase; DNA-directed RNA polymerase III subunit RPC1; DNA-directed RNA polymerase subunit; MGC62420; Polr3a; polymerase (RNA) III (DNA directed) polypeptide A; polymerase (RNA) III (DNA directed) polypeptide A, 155kDa; RPC1; RPC155
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Gene Symbols: Polr3a
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Molecular weight:
155,738 Da
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Basal Isoelectric point:
8.78
Predict pI for various phosphorylation states
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Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology®
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