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RBM21
Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation. Belongs to the DNA polymerase type-B-like family. Widely expressed. Note: This description may include information from UniProtKB.
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| Protein type: EC 2.7.7.19; EC 2.7.7.52; Nucleolus; Transferase |
| Chromosomal Location of mouse Ortholog: 19|19 A |
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Cellular Component:
cytosol; mitochondrion; mRNA cleavage and polyadenylation specificity factor complex; nuclear speck; nucleolus; nucleoplasm; nucleus
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Molecular Function:
ATP binding; enzyme binding; enzyme-substrate adaptor activity; metal ion binding; mRNA 3'-UTR binding; nucleic acid binding; nucleotide binding; nucleotidyltransferase activity; poly(A) RNA polymerase activity; RNA binding; RNA uridylyltransferase activity; transferase activity; U6 snRNA binding; zinc ion binding
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Biological Process:
mRNA polyadenylation; mRNA processing; snRNA processing; U6 snRNA 3'-end processing
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Reference #:
Q8R3F9
(UniProtKB)
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Alt. Names/Synonyms: 2700038E08Rik; PAPD2; Rbm2; Rbm21; RNA binding motif protein 21; RNA-binding motif protein 21; RNA-binding protein 21; Speckle targeted PIP5K1A-regulated poly(A) polymerase; Star-PAP; STPAP; Tent; Tent1; terminal uridylyl transferase 1, U6 snRNA-specific; Tut1; TUTas; TUTase6; U6 snRNA-specific terminal uridylyltransferase 1; U6-TUTase
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Gene Symbols: Tut1
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Molecular weight:
94,603 Da
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Basal Isoelectric point:
5.56
Predict pI for various phosphorylation states
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