eNOS Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. Belongs to the NOS family. Platelets, placenta, liver and kidney. 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Amino Acid Metabolism - arginine and proline; EC; Oxidoreductase
Chromosomal Location of mouse Ortholog: 5 A3|5 11.32 cM
Cellular Component:  apical part of cell; caveola; cytoplasm; cytoskeleton; cytosol; Golgi apparatus; membrane; membrane raft; mitochondrion; nucleolus; nucleus; plasma membrane; sarcolemma; vesicle membrane
Molecular Function:  actin binding; actin monomer binding; arginine binding; beta-catenin binding; cadherin binding; calmodulin binding; flavin adenine dinucleotide binding; FMN binding; heme binding; Hsp90 protein binding; metal ion binding; NADP binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; nitric-oxide synthase binding; oxidoreductase activity; scaffold protein binding; tetrahydrobiopterin binding
Biological Process:  angiogenesis; aortic valve morphogenesis; arginine catabolic process; blood vessel remodeling; cellular response to mechanical stimulus; cellular response to transforming growth factor beta stimulus; endocardial cushion morphogenesis; endothelial cell migration; homeostasis of number of cells within a tissue; in utero embryonic development; lipopolysaccharide-mediated signaling pathway; lung development; negative regulation of biomineral tissue development; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of hydrolase activity; negative regulation of muscle hyperplasia; negative regulation of potassium ion transport; negative regulation of smooth muscle cell proliferation; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; ovulation from ovarian follicle; positive regulation of angiogenesis; positive regulation of apoptotic process; positive regulation of blood vessel endothelial cell migration; positive regulation of gene expression; positive regulation of guanylate cyclase activity; pulmonary valve morphogenesis; regulation of blood vessel size; regulation of neurological system process; regulation of sodium ion transport; regulation of systemic arterial blood pressure by endothelin; regulation of the force of heart contraction by chemical signal; removal of superoxide radicals; response to estradiol; response to hormone; response to lipopolysaccharide; signal transduction; smooth muscle hyperplasia; ventricular septum morphogenesis
Reference #:  P70313 (UniProtKB)
Alt. Names/Synonyms: 2310065A03Rik; cNOS; Constitutive NOS; EC-NOS; Ecnos; endothelial nitric oxide synthase; Endothelial NOS; eNOS; nitric oxide synthase 3, endothelial cell; Nitric oxide synthase, endothelial; NOS type III; Nos-3; Nos3; NOSIII; OTTMUSP00000028591
Gene Symbols: Nos3
Molecular weight: 132,916 Da
Basal Isoelectric point: 6.61  Predict pI for various phosphorylation states
CST Pathways:  AMPK Signaling  |  Angiogenesis  |  PI3K/Akt Signaling
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  Reactome  |  BioGPS  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene