RTF1 Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex. Note: This description may include information from UniProtKB.
Protein type: Transcription factor
Chromosomal Location of mouse Ortholog: 2|2 E5
Cellular Component:  Cdc73/Paf1 complex; nucleolus; nucleus
Molecular Function:  DNA binding; protein binding; RNA polymerase II C-terminal domain phosphoserine binding; single-stranded DNA binding
Biological Process:  blastocyst growth; endodermal cell fate commitment; histone H3-K4 trimethylation; negative regulation of transcription by RNA polymerase II; positive regulation of histone H3-K4 methylation; stem cell population maintenance; transcription elongation by RNA polymerase II promoter; Wnt signaling pathway
Reference #:  A2AQ19 (UniProtKB)
Alt. Names/Synonyms: 2900005O08Rik; 6530416A09Rik; AI853581; AU043144; AW553985; cbp82; gene trap locus 7; Gtl; Gtl7; Paf1/RNA polymerase II complex component; RNA polymerase-associated protein RTF1 homolog; Rtf1; Rtf1 Paf1/RNA polymerase II complex component homolog; Rtf1 Paf1/RNA polymerase II complex component homolog (S. cerevisiae); RTF1, Paf1/RNA polymerase II complex component; Rtf1, Paf1/RNA polymerase II complex component, homolog; Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Gene Symbols: Rtf1
Molecular weight: 80,797 Da
Basal Isoelectric point: 8.22  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  BioGPS  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene