RNF20 Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation. Belongs to the BRE1 family. Expressed in the normal brain and also in malignant gliomas (at protein level). Note: This description may include information from UniProtKB.
Protein type: EC 6.3.2.-; EC; Ligase; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of mouse Ortholog: 4|4 B1
Cellular Component:  HULC complex; nucleolus; nucleoplasm; nucleus; ubiquitin ligase complex
Molecular Function:  chromatin binding; histone binding; identical protein binding; metal ion binding; mRNA 3'-UTR binding; p53 binding; protein binding; transcription coactivator activity; transferase activity; ubiquitin protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein transferase activity
Biological Process:  chromatin organization; histone H2B conserved C-terminal lysine ubiquitination; histone H2B ubiquitination; histone monoubiquitination; histone ubiquitination; negative regulation of cell migration; positive regulation of DNA-templated transcription; positive regulation of histone methylation; protein polyubiquitination; regulation of DNA-templated transcription; ubiquitin-dependent protein catabolic process
Reference #:  Q5DTM8 (UniProtKB)
Alt. Names/Synonyms: 4833430L21Rik; AW540162; BRE1-A; Bre1a; C79397; E3 ubiquitin-protein ligase BRE1A; Kiaa4116; mKIAA4116; OTTMUSP00000007359; RING finger protein 20; RING-type E3 ubiquitin transferase BRE1A; Rnf20
Gene Symbols: Rnf20
Molecular weight: 113,520 Da
Basal Isoelectric point: 5.74  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  BioGPS  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene