SEN2 Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity. Belongs to the tRNA-intron endonuclease family. Isoform 1 and isoform 2 are widely expressed at very low level. 5 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: EC; Nucleolus; RNA processing; Ribonuclease
Chromosomal Location of human Ortholog: 3p25.2
Cellular Component:  centrosome; cytosol; nucleolus; nucleoplasm; tRNA-intron endonuclease complex
Molecular Function:  lyase activity; nucleic acid binding; protein binding; tRNA-intron endonuclease activity
Biological Process:  mRNA processing; tRNA splicing, via endonucleolytic cleavage and ligation; tRNA-type intron splice site recognition and cleavage
Disease: Pontocerebellar Hypoplasia, Type 2b
Reference #:  Q8NCE0 (UniProtKB)
Alt. Names/Synonyms: HsSen2; MGC2776; MGC4440; PCH2B; SEN2; SEN2L; tRNA splicing endonuclease 2 homolog (S. cerevisiae); tRNA splicing endonuclease subunit 2; tRNA-intron endonuclease Sen2; tRNA-splicing endonuclease subunit Sen2; TSEN2; TSEN2 tRNA splicing endonuclease subunit
Gene Symbols: TSEN2
Molecular weight: 53,247 Da
Basal Isoelectric point: 7.95  Predict pI for various phosphorylation states
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Protein Structure Not Found.

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