RHOA Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such cytoskeletal dynamics, cell migration and cell cycle. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis. May be an activator of PLCE1. In neurons, involved in the inhibiton of the initial spine growth. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling. (Microbial infection) Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague. Belongs to the small GTPase superfamily. Rho family. Note: This description may include information from UniProtKB.
Protein type: G protein; G protein, monomeric; G protein, monomeric, Rho; Motility/polarity/chemotaxis; Oncoprotein
Chromosomal Location of human Ortholog: 3p21.31
Cellular Component:  apical junction complex; axon; cell cortex; cell junction; cell periphery; cytoskeleton; cytosol; dendritic spine; endoplasmic reticulum membrane; endosome; extrinsic component of cytoplasmic side of plasma membrane; ficolin-1-rich granule membrane; glutamatergic synapse; lamellipodium; midbody; nucleus; plasma membrane; postsynapse; ruffle membrane; secretory granule membrane; vesicle
Molecular Function:  GDP binding; GTP binding; GTPase activity; myosin binding; protein binding; protein domain specific binding; Rho GDP-dissociation inhibitor binding
Biological Process:  actin cytoskeleton organization; actin cytoskeleton reorganization; alpha-beta T cell lineage commitment; androgen receptor signaling pathway; angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure; apical junction assembly; apolipoprotein A-I-mediated signaling pathway; beta selection; cell junction assembly; cell migration; cell-matrix adhesion; cellular response to chemokine; cellular response to cytokine stimulus; cellular response to lipopolysaccharide; cerebral cortex cell migration; cleavage furrow formation; endothelial cell migration; endothelial tube lumen extension; ephrin receptor signaling pathway; establishment of epithelial cell apical/basal polarity; forebrain radial glial cell differentiation; G protein-coupled receptor signaling pathway; GTP metabolic process; mitotic cleavage furrow formation; mitotic spindle assembly; negative chemotaxis; negative regulation of axonogenesis; negative regulation of cell migration involved in sprouting angiogenesis; negative regulation of cell size; negative regulation of cell-substrate adhesion; negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of intracellular steroid hormone receptor signaling pathway; negative regulation of neuron apoptotic process; negative regulation of oxidative phosphorylation; negative regulation of reactive oxygen species biosynthetic process; neuron projection morphogenesis; neutrophil degranulation; odontogenesis; ossification involved in bone maturation; phosphatidylinositol-mediated signaling; positive regulation of actin filament polymerization; positive regulation of alpha-beta T cell differentiation; positive regulation of axonogenesis; positive regulation of cell growth; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of cytokinesis; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of leukocyte adhesion to vascular endothelial cell; positive regulation of lipase activity; positive regulation of neuron apoptotic process; positive regulation of neuron differentiation; positive regulation of NIK/NF-kappaB signaling; positive regulation of podosome assembly; positive regulation of protein serine/threonine kinase activity; positive regulation of stress fiber assembly; positive regulation of T cell migration; positive regulation of translation; positive regulation of vascular smooth muscle contraction; protein deubiquitination; regulation of actin cytoskeleton organization; regulation of calcium ion transport; regulation of cell migration; regulation of cell motility; regulation of dendrite development; regulation of focal adhesion assembly; regulation of microtubule cytoskeleton organization; regulation of modification of postsynaptic actin cytoskeleton; regulation of neural precursor cell proliferation; regulation of osteoblast proliferation; regulation of small GTPase mediated signal transduction; regulation of systemic arterial blood pressure by endothelin; regulation of transcription by RNA polymerase II; response to amino acid; response to ethanol; response to glucocorticoid; response to glucose; response to hypoxia; response to mechanical stimulus; Rho protein signal transduction; Roundabout signaling pathway; skeletal muscle satellite cell migration; skeletal muscle tissue development; stress fiber assembly; stress-activated protein kinase signaling cascade; trabecula morphogenesis; transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; viral process; Wnt signaling pathway, planar cell polarity pathway; wound healing, spreading of cells
Disease: Ectodermal Dysplasia With Facial Dysmorphism And Acral, Ocular, And Brain Anomalies
Reference #:  P61586 (UniProtKB)
Alt. Names/Synonyms: Aplysia ras-related homolog 12; ARH12; ARHA; EDFAOB; epididymis secretory sperm binding protein; h12; oncogene RHO H12; ras homolog family member A; ras homolog gene family, member A; Rho cDNA clone 12; RHO12; RHOA; RHOH12; small GTP binding protein RhoA; Transforming protein RhoA
Gene Symbols: RHOA
Molecular weight: 21,768 Da
Basal Isoelectric point: 5.83  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  Adherens Junction Dynamics  |  B Cell Receptor Signaling  |  Microtubule Dynamics  |  SAPK/JNK Signaling Cascades  |  TGF-ß Signaling
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  cBioPortal  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene