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DDX1
Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1. Specifically binds (via helicase ATP-binding domain) on both short and long poly(I:C) dsRNA. (Microbial infection) Required for HIV-1 Rev function as well as for HIV-1 and coronavirus IBV replication. Binds to the RRE sequence of HIV-1 mRNAs. (Microbial infection) Required for Coronavirus IBV replication. Belongs to the DEAD box helicase family. DDX1 subfamily. Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord. 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
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| Protein type: EC 3.6.4.13; Helicase; RNA-binding |
| Chromosomal Location of human Ortholog: 2p24.3 |
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Cellular Component:
cleavage body; cytoplasm; cytoplasmic stress granule; cytosol; mitochondrion; nucleoplasm; nucleus; ribonucleoprotein complex; tRNA-splicing ligase complex
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Molecular Function:
ATP binding; ATP hydrolysis activity; chromatin binding; DNA binding; DNA/RNA helicase activity; double-stranded RNA binding; exonuclease activity; nuclease activity; poly(A) binding; protein binding; RNA helicase activity; transcription coregulator activity
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Biological Process:
defense response to virus; DNA duplex unwinding; DNA-templated transcription; double-strand break repair; innate immune response; nucleic acid phosphodiester bond hydrolysis; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of myeloid dendritic cell cytokine production; protein localization to cytoplasmic stress granule; regulation of translational initiation; response to exogenous dsRNA; spliceosomal complex assembly; tRNA splicing, via endonucleolytic cleavage and ligation
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Reference #:
Q92499
(UniProtKB)
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Alt. Names/Synonyms: ATP-dependent RNA helicase DDX1; DBP-RB; DDX1; DEAD (Asp-Glu-Ala-Asp) box helicase 1; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD box-1; DEAD-box helicase 1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1; DEAD/H-box helicase 1; UKVH5d
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Gene Symbols: DDX1
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Molecular weight:
82,432 Da
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Basal Isoelectric point:
6.8
Predict pI for various phosphorylation states
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