SH3RF1 Has E3 ubiquitin-protein ligase activity. In the absence of an external substrate, it can catalyze self-ubiquitination. Stimulates ubiquitination of potassium channel KCNJ1, enhancing it's dynamin-dependent and clathrin-independent endocytosis. Acts as a scaffold protein that coordinates with MAPK8IP1/JIP1 in organizing different components of the JNK pathway, including RAC1 or RAC2, MAP3K11/MLK3 or MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and/or MAPK9/JNK2 into a functional multiprotein complex to ensure the effective activation of the JNK signaling pathway. Regulates the differentiation of CD4(+) and CD8(+) T-cells and promotes T-helper 1 (Th1) cell differentiation. Regulates the activation of MAPK8/JNK1 and MAPK9/JNK2 in CD4(+) T-cells and the activation of MAPK8/JNK1 in CD8(+) T-cells. Plays a crucial role in the migration of neocortical neurons in the developing brain. Controls proper cortical neuronal migration and the formation of proximal cytoplasmic dilation in the leading process (PCDLP) in migratory neocortical neurons by regulating the proper localization of activated RAC1 and F-actin assembly. (Microbial infection) Plays an essential role in the targeting of HIV-1 Gag to the plasma membrane, this function is dependent on it's RING domain, and hence it's E3 ligase activity. Belongs to the SH3RF family. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold; EC 6.3.2.-; EC; Ligase; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 8|8 B3.1
Cellular Component:  cell projection; cytoplasm; cytosol; Golgi apparatus; lamellipodium
Molecular Function:  MAP-kinase scaffold activity; metal ion binding; protein binding; transferase activity; ubiquitin protein ligase activity
Biological Process:  apoptotic process; negative regulation of apoptotic process; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; negative regulation of extrinsic apoptotic signaling pathway; neuron migration; positive regulation of JNK cascade; protein autoubiquitination; regulation of CD4-positive, alpha-beta T cell differentiation; regulation of CD8-positive, alpha-beta T cell proliferation; regulation of JNK cascade
Reference #:  Q69ZI1 (UniProtKB)
Alt. Names/Synonyms: 2200003J05Rik; E3 ubiquitin-protein ligase SH3RF1; Kiaa1494; plenty of SH3 domains; Plenty of SH3s; Posh; Posh1; Protein POSH; Putative E3 ubiquitin-protein ligase SH3RF1; R75531; RING-type E3 ubiquitin transferase SH3RF1; SH3 domain containing ring finger 1; SH3 domain-containing RING finger protein 1; SH3 multiple domains 2; SH3 multiple domains protein 2; Sh3md; Sh3md2; SH3R1; Sh3rf1
Gene Symbols: Sh3rf1
Molecular weight: 93,447 Da
Basal Isoelectric point: 8.81  Predict pI for various phosphorylation states
CST Pathways:  SAPK/JNK Signaling Cascades
Select Structure to View Below


Protein Structure Not Found.

Cross-references to other databases:  STRING  |  BioGPS  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene