Isoelectric Point Calculator
NCK2 (human)

pI for unphosphorylated state: 6.49
Number of Phosphorylated Residues to show: 1 through

# of residues pI
1 6.28
2 6.11
3 5.97
4 5.84
5 5.72
6 5.61
7 5.51
8 5.42
9 5.34
10 5.27
11 5.19
12 5.13
13 5.07
14 5.01
15 4.96
16 4.91
17 4.86
18 4.82
19 4.78
20 4.73
21 4.69
22 4.65
23 4.62
24 4.58
25 4.54
26 4.51
27 4.47

The molecular weights here are derived from the 1997 IUPAC standard atomic weights, assuming pH = 7.0. The isoelectric points are calculated using the algorithm from ExPASy's Compute pI/Mw program, which was kindly provided by Elisabeth Gasteiger (see also Bjellqvist, B., Hughes, G.J., Pasquali, Ch., Paquet, N., Ravier, F., Sanchez, J.-Ch., Frutiger, S. & Hochstrasser, D.F., The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences, Electrophoresis 1993, 14:1023-1031). We have added the option to include phosphorylations, using pKa = 2.12 for the first ionization and pKa = 7.21 for the second.