Jang SW, et al. (2009) Interaction of Akt-phosphorylated SRPK2 with 14-3-3 mediates cell cycle and cell death in neurons. J Biol Chem 284, 24512-25 19592491

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S1180-p - acinus (human) ▲
Modsite: GPRsRsRsRDRRRKE SwissProt Entrez-Gene Orthologous residues acinus (human): S1180‑p, acinus iso2 (human): S453‑p, acinus iso5 (human): S1167‑p, acinus (mouse): S1179‑p, acinus iso4 (mouse): S1139‑p, acinus iso5 (mouse): S393‑p, acinus (rat): S1180‑p, acinus (cow): S1175‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE SRPK2 (human)

S473-p - Akt1 (human) ▲

Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene Orthologous residues Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EGF increase LY294002 EGF inhibit treatment-induced increase wortmannin EGF inhibit treatment-induced increase GF109203X EGF no effect upon treatment-induced increase PD98059 EGF no effect upon treatment-induced increase

T492-p - SRPK2 (human) ▲

Modsite: PSHDRSRtVsAsstG SwissProt Entrez-Gene Orthologous residues SRPK2 (human): T492‑p, SRPK2 iso2 (human): T503‑p, SRPK2 (mouse): T485‑p, SRPK2 (rat): T485‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Akt1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Akt1 (human) pharmacological inhibitor of upstream enzyme, phospho-motif antibody, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EGF increase LY294002 EGF inhibit treatment-induced increase wortmannin EGF inhibit treatment-induced increase GF109203X EGF no effect upon treatment-induced increase PD98059 EGF no effect upon treatment-induced increase Downstream Regulation Effect of modification (function):  enzymatic activity, induced, intracellular localization, molecular association, regulation Effect of modification (process):  cell cycle regulation, transcription, altered Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays 14-3-3 epsilon (human) Induces co-immunoprecipitation 14-3-3 beta (human) Induces co-immunoprecipitation Comments:  nuclear translocation of SRPK2 is blocked by 14-3-3

S473-p - Akt1 (mouse) ▲

Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene Orthologous residues Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'neuron, cortical' Cellular systems studied:  cell lines, primary cultured cells Species studied:  mouse, rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes ischemia increase

T485-p - SRPK2 (mouse) ▲

Modsite: PsHDRsRtVsAsstG SwissProt Entrez-Gene Orthologous residues SRPK2 (human): T492‑p, SRPK2 iso2 (human): T503‑p, SRPK2 (mouse): T485‑p, SRPK2 (rat): T485‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'neuron, cortical' Cellular systems studied:  cell lines, primary cultured cells Species studied:  mouse, rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes ischemia increase

T485-p - SRPK2 (rat) ▲

Modsite: PSRDRSRtVsASstG GenPept Entrez-Gene Orthologous residues SRPK2 (human): T492‑p, SRPK2 iso2 (human): T503‑p, SRPK2 (mouse): T485‑p, SRPK2 (rat): T485‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'neuron, cortical' Cellular systems studied:  cell lines, primary cultured cells Species studied:  mouse, rat Downstream Regulation Effect of modification (process):  apoptosis, altered