Curated Information
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Home > Curated Information Page > PubMed Id: 19710014
Aiken CT, et al. (2009) Phosphorylation of threonine 3: implications for Huntingtin aggregation and neurotoxicity. J Biol Chem 284, 29427-36 19710014
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T3-p - Huntingtin (human)
Modsite: _____MAtLEkLMkA SwissProt Entrez-Gene
Orthologous residues
Huntingtin (human): T3‑p, Huntingtin (mouse): T3‑p, Huntingtin (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry, microscopy-colocalization with upstream kinase, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum', eye cell, HeLa (cervical), ST14A
Cellular systems studied:  cell lines, tissue
Species studied:  fruit fly, human, mouse
Comments:  transgenic Drosophila expressing wt (97Q), phosphomimetic (97QT3D) or non-phosphorylatable (97QT3A) huntingtin, wt and CAG140 mice (knock-in of human huntingtin containing 140 CAG repeats)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Huntingtin (human) Induces microscopy-colocalization
Comments:  enhances huntingtin aggregation and neurodegeneration in transgenic flies

K6-ac - Huntingtin (human)
Modsite: __MAtLEkLMkAFEs SwissProt Entrez-Gene
Orthologous residues
Huntingtin (human): K6‑ac, Huntingtin (mouse): K6‑ac, Huntingtin (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical), ST14A
Cellular systems studied:  cell lines
Species studied:  human

S13-p - Huntingtin (human)
Modsite: kLMkAFEsLksFQQQ SwissProt Entrez-Gene
Orthologous residues
Huntingtin (human): S13‑p, Huntingtin (mouse): S13‑p, Huntingtin (rat): S6‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical), ST14A
Cellular systems studied:  cell lines
Species studied:  human