Curated Information
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Home > Curated Information Page > Cst Curation Set #388
Li Y (2004) CST Curation Set: 388; Year: 2004; Biosample/Treatment: cell line, HCT116/Serum starved; Disease: colorectal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y302-p - calponin 2 (human)
Modsite: EVPEyPPyyQEEAGy SwissProt Entrez-Gene
Orthologous residues
calponin 2 (human): Y302‑p, calponin 2 iso2 (human): Y263‑p, calponin 2 (mouse): Y298‑p, calponin 2 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

Y303-p - calponin 2 (human)
Modsite: VPEyPPyyQEEAGy_ SwissProt Entrez-Gene
Orthologous residues
calponin 2 (human): Y303‑p, calponin 2 iso2 (human): Y264‑p, calponin 2 (mouse): C299‑p, calponin 2 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

Y362-p - DOK1 (human)
Modsite: DPKEDPIyDEPEGLA SwissProt Entrez-Gene
Orthologous residues
DOK1 (human): Y362‑p, DOK1 iso3 (human): Y223‑p, DOK1 (mouse): Y361‑p, DOK1 (rat): Y361‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

Y377-p - DOK1 (human)
Modsite: PVPPQGLyDLPREPK SwissProt Entrez-Gene
Orthologous residues
DOK1 (human): Y377‑p, DOK1 iso3 (human): Y238‑p, DOK1 (mouse): Y376‑p, DOK1 (rat): Y376‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

Y279-p - GSK3A (human)
Modsite: RGEPNVsyICsRyyR SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): Y279‑p, GSK3A (mouse): Y279‑p, GSK3A (rat): Y279‑p, GSK3A (cow): Y279‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

Y216-p - GSK3B (human)
Modsite: RGEPNVsyICsRyyR SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): Y216‑p, GSK3B iso2 (human): Y216‑p, GSK3B (mouse): Y216‑p, GSK3B (rat): Y216‑p, GSK3B (rabbit): Y216‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

Y352-p - HIPK1 (human)
Modsite: SkAVCstyLQsRYYR SwissProt Entrez-Gene
Orthologous residues
HIPK1 (human): Y352‑p, HIPK1 iso2 (human): Y352‑p, HIPK1 iso4 (human): , HIPK1 (mouse): Y352‑p, HIPK1 (rat): Y352‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

Y361-p - HIPK2 (human)
Modsite: SkAVCstyLQsRYyR SwissProt Entrez-Gene
Orthologous residues
HIPK2 (human): Y361‑p, HIPK2 (mouse): Y361‑p, HIPK2 (rat): Y361‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

Y153-p - hnRNP 2H9 (human)
Modsite: GyGGFDDyGGyNNyG SwissProt Entrez-Gene
Orthologous residues
hnRNP 2H9 (human): Y153‑p, hnRNP 2H9 iso2 (human): Y138‑p, hnRNP 2H9 iso3 (human): Y104‑p, hnRNP 2H9 (mouse): Y153‑p, hnRNP 2H9 (rat): Y153‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

Y246-p - hnRNP H1 (human)
Modsite: GyGGyDDyNGyNdGy SwissProt Entrez-Gene
Orthologous residues
hnRNP H1 (human): Y246‑p, hnRNP H1 (mouse): Y246‑p, hnRNP H1 (rat): Y246‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

Y796-p - VCP (human)
Modsite: GGtGGsVytEDNDDD SwissProt Entrez-Gene
Orthologous residues
VCP (human): Y796‑p, VCP (mouse): Y796‑p, VCP (rat): Y796‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

Y805-p - VCP (human)
Modsite: EDNDDDLyG______ SwissProt Entrez-Gene
Orthologous residues
VCP (human): Y805‑p, VCP (mouse): Y805‑p, VCP (rat): Y805‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human