Curated Information
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Home > Curated Information Page > Cst Curation Set #187
Guo A (2003) CST Curation Set: 187; Year: 2003; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y461-p - abLIM (human)
Modsite: GsINsPVysRHsytP SwissProt Entrez-Gene
Orthologous residues
abLIM (human): Y461‑p, abLIM iso2 (human): Y401‑p, abLIM iso3 (human): Y145‑p, abLIM iso5 (human): Y173‑p, abLIM (mouse): Y505‑p, abLIM iso2 (mouse): Y385‑p, abLIM iso3 (mouse): Y385‑p, abLIM (rat): Y145‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y173-p - abLIM iso5 (human)
Modsite: GSINSPVySRHSYtP SwissProt Entrez-Gene
Orthologous residues
abLIM (human): Y461‑p, abLIM iso2 (human): Y401‑p, abLIM iso3 (human): Y145‑p, abLIM iso5 (human): Y173‑p, abLIM (mouse): Y505‑p, abLIM iso2 (mouse): Y385‑p, abLIM iso3 (mouse): Y385‑p, abLIM (rat): Y145‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y349-p - ARF GAP 3 (human)
Modsite: NDDSDDsyFtSSssy SwissProt Entrez-Gene
Orthologous residues
ARF GAP 3 (human): Y349‑p, ARF GAP 3 (mouse): Y349‑p, ARF GAP 3 (rat): Y350‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y149-p - ATP6V0A2 (human)
Modsite: NVEFEPtyEEFPsLE SwissProt Entrez-Gene
Orthologous residues
ATP6V0A2 (human): Y149‑p, ATP6V0A2 iso2 (human): Y149‑p, ATP6V0A2 (mouse): Y149‑p, ATP6V0A2 (rat): Y149‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y163-p - ATP6V0A2 (human)
Modsite: EsDSLLDySCMQRLG SwissProt Entrez-Gene
Orthologous residues
ATP6V0A2 (human): Y163‑p, ATP6V0A2 iso2 (human): Y163‑p, ATP6V0A2 (mouse): Y163‑p, ATP6V0A2 (rat): Y163‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y149-p - ATP6V0A2 iso2 (human)
Modsite: NVEFEPTyEEFPSLE SwissProt Entrez-Gene
Orthologous residues
ATP6V0A2 (human): Y149‑p, ATP6V0A2 iso2 (human): Y149‑p, ATP6V0A2 (mouse): Y149‑p, ATP6V0A2 (rat): Y149‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y163-p - ATP6V0A2 iso2 (human)
Modsite: ESDSLLDySCMQRLG SwissProt Entrez-Gene
Orthologous residues
ATP6V0A2 (human): Y163‑p, ATP6V0A2 iso2 (human): Y163‑p, ATP6V0A2 (mouse): Y163‑p, ATP6V0A2 (rat): Y163‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y149-p - CD3D (human)
Modsite: LLRNDQVyQPLRDRD SwissProt Entrez-Gene
Orthologous residues
CD3D (human): Y149‑p, CD3D (mouse): Y149‑p, CD3D (rat): Y149‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y188-p - CD3E (human)
Modsite: PPVPNPDyEPIRKGQ SwissProt Entrez-Gene
Orthologous residues
CD3E (human): Y188‑p, CD3E (mouse): Y170‑p, CD3E (rat): Y169‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y199-p - CD3E (human)
Modsite: RKGQRDLysGLNQRR SwissProt Entrez-Gene
Orthologous residues
CD3E (human): Y199‑p, CD3E (mouse): Y181‑p, CD3E (rat): Y180‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y142-p - CD3Z (human)
Modsite: GkGHDGLyQGLstAt SwissProt Entrez-Gene
Orthologous residues
CD3Z (human): Y142‑p, CD3Z iso3 (human): Y141‑p, CD3Z (mouse): Y142‑p, CD3Z iso2 (mouse): Y142‑p, CD3Z (rat): Y142‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y453-p - CD5 (human)
Modsite: AsHVDNEysQPPRNs SwissProt Entrez-Gene
Orthologous residues
CD5 (human): Y453‑p, CD5 (mouse): Y452‑p, CD5 (rat): Y449‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y15-p - CDK1 (human)
Modsite: EkIGEGtyGVVykGR SwissProt Entrez-Gene
Orthologous residues
CDK1 (human): Y15‑p, CDK1 iso2 (human): Y15‑p, CDK1 (mouse): Y15‑p, CDK1 (rat): Y15‑p, CDK1 (chicken): Y15‑p, CDK1 (fruit fly): Y15‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y15-p - CDK2 (human)
Modsite: EkIGEGtyGVVykAR SwissProt Entrez-Gene
Orthologous residues
CDK2 (human): Y15‑p, CDK2 iso2 (human): Y15‑p, CDK2 (mouse): Y15‑p, CDK2 (rat): Y15‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y15-p - CDK3 (human)
Modsite: EkIGEGtyGVVyKAK SwissProt Entrez-Gene
Orthologous residues
CDK3 (human): Y15‑p, CDK3 (mouse): Y15‑p, CDK3 (cow): Y15‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y209-p - DOCK2 (human)
Modsite: MskDQPdyAMysRIS SwissProt Entrez-Gene
Orthologous residues
DOCK2 (human): Y209‑p, DOCK2 (mouse): Y209‑p, DOCK2 (rat): Y209‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y212-p - DOCK2 (human)
Modsite: DQPdyAMysRISSsP SwissProt Entrez-Gene
Orthologous residues
DOCK2 (human): Y212‑p, DOCK2 (mouse): Y212‑p, DOCK2 (rat): Y212‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y449-p - DOK1 (human)
Modsite: KsHNsALysQVQKSG SwissProt Entrez-Gene
Orthologous residues
DOK1 (human): Y449‑p, DOK1 iso3 (human): Y310‑p, DOK1 (mouse): Y450‑p, DOK1 (rat): Y448‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y95-p - emerin (human)
Modsite: kGyNDDyyEEsyFtT SwissProt Entrez-Gene
Orthologous residues
emerin (human): Y95‑p, emerin (mouse): Y96‑p, emerin (rat): Y96‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y105-p - emerin (human)
Modsite: syFtTrtyGEPEsAG SwissProt Entrez-Gene
Orthologous residues
emerin (human): Y105‑p, emerin (mouse): Y106‑p, emerin (rat): Y106‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y181-p - emerin (human)
Modsite: RssLDLsyyPTSSST SwissProt Entrez-Gene
Orthologous residues
emerin (human): Y181‑p, emerin (mouse): Y182‑p, emerin (rat): Y183‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y2502-p - FLNB (human)
Modsite: RSSTEtCysAIPKAS SwissProt Entrez-Gene
Orthologous residues
FLNB (human): Y2502‑p, FLNB iso2 (human): Y2478‑p, FLNB iso8 (human): Y2533‑p, FLNB (mouse): Y2502‑p, FLNB (rat): Y2478‑p, FLNB (cow): Y2533‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y420-p - Fyn (human)
Modsite: RLIEDNEytARQGAk SwissProt Entrez-Gene
Orthologous residues
Fyn (human): Y420‑p, Fyn iso2 (human): Y417‑p, Fyn iso3 (human): Y365‑p, Fyn (mouse): Y420‑p, Fyn (rat): Y420‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y1105-p - GRF-1 (human)
Modsite: RNEEENIysVPHDst SwissProt Entrez-Gene
Orthologous residues
GRF‑1 (human): Y1105‑p, GRF‑1 (mouse): Y1105‑p, GRF‑1 (rat): Y1105‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y279-p - GSK3A (human)
Modsite: RGEPNVsyICsRyyR SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): Y279‑p, GSK3A (mouse): Y279‑p, GSK3A (rat): Y279‑p, GSK3A (cow): Y279‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y216-p - GSK3B (human)
Modsite: RGEPNVsyICsRyyR SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): Y216‑p, GSK3B iso2 (human): Y216‑p, GSK3B (mouse): Y216‑p, GSK3B (rat): Y216‑p, GSK3B (rabbit): Y216‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y352-p - HIPK1 (human)
Modsite: SkAVCstyLQsRYYR SwissProt Entrez-Gene
Orthologous residues
HIPK1 (human): Y352‑p, HIPK1 iso2 (human): Y352‑p, HIPK1 iso4 (human): , HIPK1 (mouse): Y352‑p, HIPK1 (rat): Y352‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y361-p - HIPK2 (human)
Modsite: SkAVCstyLQsRYyR SwissProt Entrez-Gene
Orthologous residues
HIPK2 (human): Y361‑p, HIPK2 (mouse): Y361‑p, HIPK2 (rat): Y361‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y180-p - hnRNP A0 (human)
Modsite: AVPkEDIysGGGGGG SwissProt Entrez-Gene
Orthologous residues
hnRNP A0 (human): Y180‑p, hnRNP A0 (mouse): H180‑p, hnRNP A0 (rat): H180‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y394-p - Lck (human)
Modsite: RLIEDNEytAREGAk SwissProt Entrez-Gene
Orthologous residues
Lck (human): Y394‑p, Lck iso3 (human): Y424‑p, Lck (mouse): Y394‑p, Lck (rat): Y394‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y505-p - Lck (human)
Modsite: FTAtEGQyQPQP___ SwissProt Entrez-Gene
Orthologous residues
Lck (human): Y505‑p, Lck iso3 (human): Y535‑p, Lck (mouse): Y505‑p, Lck (rat): Y505‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y146-p - RBM3 (human)
Modsite: NQGGyDrysGGNyRD SwissProt Entrez-Gene
Orthologous residues
RBM3 (human): Y146‑p, RBM3 (mouse): Y142‑p, RBM3 iso2 (mouse): Y143‑p, RBM3 (rat): Y144‑p, RBM3 iso2 (rat): Y145‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y755-p - SLAP-130 (human)
Modsite: CRNEEGkyGyVLRSy SwissProt Entrez-Gene
Orthologous residues
SLAP‑130 (human): Y755‑p, SLAP‑130 iso2 (human): Y801‑p, SLAP‑130 iso3 (human): Y811‑p, SLAP‑130 (mouse): Y791‑p, SLAP‑130 (rat): Y802‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y757-p - SLAP-130 (human)
Modsite: NEEGkyGyVLRSyLA SwissProt Entrez-Gene
Orthologous residues
SLAP‑130 (human): Y757‑p, SLAP‑130 iso2 (human): Y803‑p, SLAP‑130 iso3 (human): Y813‑p, SLAP‑130 (mouse): Y793‑p, SLAP‑130 (rat): Y804‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y42-p - SMAP-5 (human)
Modsite: ySkQyAGyDysQQGR SwissProt Entrez-Gene
Orthologous residues
SMAP‑5 (human): Y42‑p, SMAP‑5 iso3 (human): Y42‑p, SMAP‑5 (mouse): C42‑p, SMAP‑5 (rat): C42‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y44-p - SMAP-5 (human)
Modsite: kQyAGyDysQQGRFV SwissProt Entrez-Gene
Orthologous residues
SMAP‑5 (human): Y44‑p, SMAP‑5 iso3 (human): Y44‑p, SMAP‑5 (mouse): Y44‑p, SMAP‑5 (rat): Y44‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y419-p - Src (human)
Modsite: RLIEDNEytARQGAk SwissProt Entrez-Gene
Orthologous residues
Src (human): Y419‑p, Src iso2 (human): Y425‑p, Src (mouse): Y418‑p, Src iso1 (mouse): Y424‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y261-p - SRPR (human)
Modsite: ANKEVLDystPTtNG SwissProt Entrez-Gene
Orthologous residues
SRPR (human): Y261‑p, SRPR iso2 (human): Y233‑p, SRPR (mouse): Y260‑p, SRPR (rat): Y260‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y98-p - TBCB (human)
Modsite: sGARLGEyEDVsRVE SwissProt Entrez-Gene
Orthologous residues
TBCB (human): Y98‑p, TBCB (mouse): Y98‑p, TBCB (rat): Y98‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y426-p - Yes (human)
Modsite: RLIEDNEytARQGAk SwissProt Entrez-Gene
Orthologous residues
Yes (human): Y426‑p, Yes (mouse): Y424‑p, Yes (rat): Y424‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y292-p - ZAP70 (human)
Modsite: DtLNsDGytPEPARI SwissProt Entrez-Gene
Orthologous residues
ZAP70 (human): Y292‑p, ZAP70 (mouse): Y290‑p, ZAP70 (rat): Y290‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y493-p - ZAP70 (human)
Modsite: LGADDsyytARsAGk SwissProt Entrez-Gene
Orthologous residues
ZAP70 (human): Y493‑p, ZAP70 (mouse): Y492‑p, ZAP70 (rat): Y488‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human