Curated Information
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Home > Curated Information Page > PubMed Id: 17956904
Morita M, et al. (2008) Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C {delta} at Tyr332 to form a Trimeric Complex with p66Shc in the H2O2-stimulated Cells. J Biochem 143, 31-8 17956904
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y311-p - PKCD (rat)
Modsite: TPETVGIyQGFEKKT SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y313‑p, PKCD iso2 (human): Y313‑p, PKCD (mouse): Y311‑p, PKCD iso2 (mouse): Y311‑p, PKCD (rat): Y311‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE EGFR (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
AG1478 H2O2 inhibit treatment-induced increase
PD153035 H2O2 inhibit treatment-induced increase
EGF no change compared to control

Y332-p - PKCD (rat)
Modsite: IPDNNGTyGKIWEGS SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y334‑p, PKCD iso2 (human): Y365‑p, PKCD (mouse): Y332‑p, PKCD iso2 (mouse): Y358‑p, PKCD (rat): Y332‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE EGFR (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
AG1478 H2O2 inhibit treatment-induced increase
PD153035 H2O2 inhibit treatment-induced increase
EGF no change compared to control
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SHC1 (human) Induces co-immunoprecipitation