Curated Information
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Home > Curated Information Page > PubMed Id: 17826804
Snyder GL, Galdi S, Hendrick JP, Hemmings HC (2007) General anesthetics selectively modulate glutamatergic and dopaminergic signaling via site-specific phosphorylation in vivo. Neuropharmacology 53, 619-30 17826804
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S119-p - CREB (mouse)
Modsite: EILsRRPsyRkILND SwissProt Entrez-Gene
Orthologous residues
CREB (human): S119‑p, CREB iso1 (human): S133‑p, CREB (mouse): S119‑p, CREB iso1 (mouse): S133‑p, CREB (rat): S119‑p, CREB iso1 (rat): S133‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
propofol decrease in striatum and cortex
isoflurane no change compared to control in striatum
ketamine no change compared to control in striatum
ketamine decrease in cortex

T34-p - DARPP-32 (mouse)
Modsite: MIRRRRPtPAMLFRV SwissProt Entrez-Gene
Orthologous residues
DARPP‑32 (human): T34‑p, DARPP‑32 iso2 (human): , DARPP‑32 (mouse): T34‑p, DARPP‑32 (rat): T34‑p, DARPP‑32 (cow): T34‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ketamine increase in striatum
ketamine no change compared to control in cortex
isoflurane decrease in striatum
isoflurane no change compared to control in cortex
propofol no change compared to control in striatum
propofol decrease in cortex
PCP increase sub-anesthetic dose in striatum
ketamine increase anesthetic dose in striatum

T75-p - DARPP-32 (mouse)
Modsite: RPNPCAYtPPSLKAV SwissProt Entrez-Gene
Orthologous residues
DARPP‑32 (human): T75‑p, DARPP‑32 iso2 (human): T39‑p, DARPP‑32 (mouse): T75‑p, DARPP‑32 (rat): T75‑p, DARPP‑32 (cow): T75‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoflurane no change compared to control in striatum and cortex
propofol no change compared to control in striatum
propofol decrease in cortex
ketamine no change compared to control in striatum
ketamine decrease in cortex

S97-p - DARPP-32 (mouse)
Modsite: NLsENQAsEEEDELG SwissProt Entrez-Gene
Orthologous residues
DARPP‑32 (human): S102‑p, DARPP‑32 iso2 (human): S66‑p, DARPP‑32 (mouse): S97‑p, DARPP‑32 (rat): S102‑p, DARPP‑32 (cow): S102‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, striatum'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
propofol no change compared to control
isoflurane decrease
ketamine no change compared to control

S130-p - DARPP-32 (mouse)
Modsite: EEDEEEDsQAEVLKG SwissProt Entrez-Gene
Orthologous residues
DARPP‑32 (human): S137‑p, DARPP‑32 iso2 (human): S101‑p, DARPP‑32 (mouse): S130‑p, DARPP‑32 (rat): S137‑p, DARPP‑32 (cow): S135‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, striatum'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
propofol no change compared to control
isoflurane no change compared to control
ketamine no change compared to control

T183-p - ERK2 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
propofol decrease
isoflurane decrease
ketamine decrease
propofol augment treatment-induced decrease anesthetic doses

S849-p - GluR1 (mouse)
Modsite: FCLIPQQsINEAIRt SwissProt Entrez-Gene
Orthologous residues
GluR1 (human): S849‑p, GluR1 iso6 (human): S859‑p, GluR1 (mouse): S849‑p, GluR1 (rat): S849‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoflurane decrease in striatum and cortex
propofol decrease in striatum
propofol no change compared to control in cortex
ketamine no change compared to control in striatum
ketamine decrease in cortex

S863-p - GluR1 (mouse)
Modsite: tstLPRNsGAGASGG SwissProt Entrez-Gene
Orthologous residues
GluR1 (human): S863‑p, GluR1 iso6 (human): S873‑p, GluR1 (mouse): S863‑p, GluR1 (rat): S863‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoflurane no change compared to control in striatum and cortex
propofol no change compared to control in striatum
propofol decrease in cortex
ketamine increase in striatum
ketamine no change compared to control in cortex

S897-p - NMDAR1 (mouse)
Modsite: sFKRRRssKDTSTGG SwissProt Entrez-Gene
Orthologous residues
NMDAR1 (human): S897‑p, NMDAR1 iso2 (human): , NMDAR1 iso5 (human): S918‑p, NMDAR1 (mouse): S897‑p, NMDAR1 iso2 (mouse): , NMDAR1 iso3 (mouse): S918‑p, NMDAR1 (rat): S897‑p, NMDAR1 iso7 (rat):
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
propofol decrease
isoflurane decrease
ketamine decrease
ketamine augment treatment-induced decrease anesthetic doses
PCP decrease sub-anesthetic doses in striatum

S94-p - Spinophilin (mouse)
Modsite: SDRGVRLsLPRAssL SwissProt Entrez-Gene
Orthologous residues
Spinophilin (human): S94‑p, Spinophilin (mouse): S94‑p, Spinophilin (rat): S94‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoflurane decrease
propofol decrease
ketamine no change compared to control

S40-p - TH (mouse)
Modsite: RFIGRRQsLIEDARK SwissProt Entrez-Gene
Orthologous residues
TH (human): S71‑p, TH iso2 (human): S67‑p, TH iso3 (human): S40‑p, TH iso4 (human): S44‑p, TH (mouse): S40‑p, TH (rat): S40‑p, TH (cow): S40‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, striatum'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoflurane increase
ketamine decrease
propofol no change compared to control