Snyder GL, Galdi S, Hendrick JP, Hemmings HC (2007) General anesthetics selectively modulate glutamatergic and dopaminergic signaling via site-specific phosphorylation in vivo. Neuropharmacology 53, 619-30 17826804

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S119-p - CREB (mouse) ▲

Modsite: EILsRRPsyRkILND SwissProt Entrez-Gene Orthologous residues CREB (human): S119‑p, CREB iso1 (human): S133‑p, CREB (mouse): S119‑p, CREB iso1 (mouse): S133‑p, CREB (rat): S133‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes propofol decrease in striatum and cortex isoflurane no change compared to control in striatum ketamine no change compared to control in striatum ketamine decrease in cortex

T34-p - DARPP-32 (mouse) ▲

Modsite: MIRRRRPtPAMLFRV SwissProt Entrez-Gene Orthologous residues DARPP‑32 (human): T34‑p, DARPP‑32 iso2 (human): ‑, DARPP‑32 (mouse): T34‑p, DARPP‑32 (rat): T34‑p, DARPP‑32 (cow): T34‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes ketamine increase in striatum ketamine no change compared to control in cortex isoflurane decrease in striatum isoflurane no change compared to control in cortex propofol no change compared to control in striatum propofol decrease in cortex PCP increase sub-anesthetic dose in striatum ketamine increase anesthetic dose in striatum

T75-p - DARPP-32 (mouse) ▲

Modsite: RPNPCAYtPPSLKAV SwissProt Entrez-Gene Orthologous residues DARPP‑32 (human): T75‑p, DARPP‑32 iso2 (human): T39‑p, DARPP‑32 (mouse): T75‑p, DARPP‑32 (rat): T75‑p, DARPP‑32 (cow): T75‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes isoflurane no change compared to control in striatum and cortex propofol no change compared to control in striatum propofol decrease in cortex ketamine no change compared to control in striatum ketamine decrease in cortex

S97-p - DARPP-32 (mouse) ▲

Modsite: NLsENQAsEEEDELG SwissProt Entrez-Gene Orthologous residues DARPP‑32 (human): S102‑p, DARPP‑32 iso2 (human): S66‑p, DARPP‑32 (mouse): S97‑p, DARPP‑32 (rat): S102‑p, DARPP‑32 (cow): S102‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes propofol no change compared to control isoflurane decrease ketamine no change compared to control

S130-p - DARPP-32 (mouse) ▲

Modsite: EEDEEEDsQAEVLKG SwissProt Entrez-Gene Orthologous residues DARPP‑32 (human): S137‑p, DARPP‑32 iso2 (human): S101‑p, DARPP‑32 (mouse): S130‑p, DARPP‑32 (rat): S137‑p, DARPP‑32 (cow): S135‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes propofol no change compared to control isoflurane no change compared to control ketamine no change compared to control

T183-p - ERK2 (mouse) ▲

Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene Orthologous residues ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes propofol decrease isoflurane decrease ketamine decrease propofol augment treatment-induced decrease anesthetic doses

S849-p - GluR1 (mouse) ▲

Modsite: FCLIPQQsINEAIRt SwissProt Entrez-Gene Orthologous residues GluR1 (human): S849‑p, GluR1 iso6 (human): S859‑p, GluR1 (mouse): S849‑p, GluR1 (rat): S849‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes isoflurane decrease in striatum and cortex propofol decrease in striatum propofol no change compared to control in cortex ketamine no change compared to control in striatum ketamine decrease in cortex

S863-p - GluR1 (mouse) ▲

Modsite: tstLPRNsGAGASGG SwissProt Entrez-Gene Orthologous residues GluR1 (human): S863‑p, GluR1 iso6 (human): S873‑p, GluR1 (mouse): S863‑p, GluR1 (rat): S863‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes isoflurane no change compared to control in striatum and cortex propofol no change compared to control in striatum propofol decrease in cortex ketamine increase in striatum ketamine no change compared to control in cortex

S897-p - NMDAR1 (mouse) ▲

Modsite: sFKRRRssKDTSTGG SwissProt Entrez-Gene Orthologous residues NMDAR1 (human): S897‑p, NMDAR1 iso2 (human): ‑, NMDAR1 iso5 (human): S918‑p, NMDAR1 (mouse): S897‑p, NMDAR1 iso2 (mouse): ‑, NMDAR1 iso3 (mouse): S918‑p, NMDAR1 (rat): S897‑p, NMDAR1 iso7 (rat): ‑ Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes propofol decrease isoflurane decrease ketamine decrease ketamine augment treatment-induced decrease anesthetic doses PCP decrease sub-anesthetic doses in striatum

S94-p - Spinophilin (mouse) ▲

Modsite: SDRGVRLsLPRAssL SwissProt Entrez-Gene Orthologous residues Spinophilin (human): S94‑p, Spinophilin (mouse): S94‑p, Spinophilin (rat): S94‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes isoflurane decrease propofol decrease ketamine no change compared to control

S40-p - TH (mouse) ▲

Modsite: RFIGRRQsLIEDARK SwissProt Entrez-Gene Orthologous residues TH (human): S71‑p, TH iso2 (human): S67‑p, TH iso3 (human): S40‑p, TH iso4 (human): S44‑p, TH (mouse): S40‑p, TH (rat): S40‑p, TH (cow): S40‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, striatum' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes isoflurane increase ketamine decrease propofol no change compared to control