Curated Information
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Home > Curated Information Page > Cst Curation Set #158
Moritz A (2003) CST Curation Set: 158; Year: 2003; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y132-p - ACP1 (mouse)
Modsite: QLIIEDPyyGNDSDF SwissProt Entrez-Gene
Orthologous residues
ACP1 (human): Y132‑p, ACP1 iso2 (human): Y132‑p, ACP1 (mouse): Y132‑p, ACP1 iso2 (mouse): Y132‑p, ACP1 (rat): Y132‑p, ACP1 (cow): Y132‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y73-p - AKAP2 (mouse)
Modsite: DSSVDGTyNGISSPE SwissProt Entrez-Gene
Orthologous residues
AKAP2 (human): Y73‑p, AKAP2 iso4 (human): Y304‑p, AKAP2 iso6 (human): Y304‑p, AKAP2 (mouse): Y73‑p, AKAP2 iso2 (mouse): Y73‑p, AKAP2 iso3 (mouse): Y73‑p, AKAP2 iso6 (mouse): Y114‑p, AKAP2 (rat): Y73‑p, AKAP2 (cow): Y73‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y24-p - ANXA2 (mouse)
Modsite: HstPPsAyGsVKPyt SwissProt Entrez-Gene
Orthologous residues
ANXA2 (human): Y24‑p, ANXA2 iso2 (human): Y42‑p, ANXA2 (mouse): Y24‑p, ANXA2 (rat): Y24‑p, ANXA2 (dog): Y24‑p, ANXA2 (cow): Y24‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y460-p - Cortactin (mouse)
Modsite: GSQQGLTyTSEPVyE SwissProt Entrez-Gene
Orthologous residues
Cortactin (human): Y464‑p, Cortactin iso2 (human): Y427‑p, Cortactin iso3 (human): Y427‑p, Cortactin (mouse): Y460‑p, Cortactin (rat): Y422‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y466-p - Cortactin (mouse)
Modsite: TyTSEPVyETTEAPG SwissProt Entrez-Gene
Orthologous residues
Cortactin (human): Y470‑p, Cortactin iso2 (human): Y433‑p, Cortactin iso3 (human): Y433‑p, Cortactin (mouse): Y466‑p, Cortactin (rat): Y428‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y44-p - ENO1 (mouse)
Modsite: sGAstGIyEALELRD SwissProt Entrez-Gene
Orthologous residues
ENO1 (human): Y44‑p, ENO1 (mouse): Y44‑p, ENO1 (rat): Y44‑p, ENO1 (chicken): Y44‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y44-p - ENO2 (mouse)
Modsite: sGAstGIyEALELRD SwissProt Entrez-Gene
Orthologous residues
ENO2 (human): Y44‑p, ENO2 (mouse): Y44‑p, ENO2 (rat): Y44‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y483-p - Ezrin (mouse)
Modsite: PVyEPVNyHVQEGLQ SwissProt Entrez-Gene
Orthologous residues
Ezrin (human): Y483‑p, Ezrin (mouse): Y483‑p, Ezrin (rat): Y483‑p, Ezrin (chicken): Y482‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y531-p - Fyn (mouse)
Modsite: FTATEPQyQPGENL_ SwissProt Entrez-Gene
Orthologous residues
Fyn (human): Y531‑p, Fyn iso2 (human): Y528‑p, Fyn iso3 (human): Y476‑p, Fyn (mouse): Y531‑p, Fyn (rat): Y531‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y607-p - PIK3R1 (mouse)
Modsite: NENTEDQysLVEDDE SwissProt Entrez-Gene
Orthologous residues
PIK3R1 (human): Y607‑p, PIK3R1 iso2 (human): Y337‑p, PIK3R1 iso4 (human): Y615‑p, PIK3R1 (mouse): Y607‑p, PIK3R1 (rat): Y607‑p, PIK3R1 (cow): Y607‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y13-p - PTRF (mouse)
Modsite: LHIVERPysGFPDAs SwissProt Entrez-Gene
Orthologous residues
PTRF (human): L13‑p, PTRF iso2 (human): L13‑p, PTRF (mouse): Y13‑p, PTRF iso3 (mouse): Y13‑p, PTRF (rat): Y13‑p, PTRF (cow): L13‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y31-p - PXN (mouse)
Modsite: FLsEEPPysyPTGNH SwissProt Entrez-Gene
Orthologous residues
PXN (human): Y31‑p, PXN iso2 (human): Y31‑p, PXN iso3 (human): Y31‑p, PXN (mouse): Y31‑p, PXN iso2 (mouse): Y31‑p, PXN (rat): Y31‑p, PXN (chicken): Y31‑p, PXN (cow): Y26‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y40-p - PXN (mouse)
Modsite: yPTGNHtyQEIAVPP SwissProt Entrez-Gene
Orthologous residues
PXN (human): Y40‑p, PXN iso2 (human): Y40‑p, PXN iso3 (human): Y40‑p, PXN (mouse): Y40‑p, PXN iso2 (mouse): Y40‑p, PXN (rat): Y40‑p, PXN (chicken): Y40‑p, PXN (cow): Y35‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y226-p - SH3PXD2B (mouse)
Modsite: QPEEEEKyTVIyPYT SwissProt Entrez-Gene
Orthologous residues
SH3PXD2B (human): Y226‑p, SH3PXD2B (mouse): Y226‑p, SH3PXD2B (rat): Y123‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y230-p - SH3PXD2B (mouse)
Modsite: EEKyTVIyPYTARDQ SwissProt Entrez-Gene
Orthologous residues
SH3PXD2B (human): Y230‑p, SH3PXD2B (mouse): Y230‑p, SH3PXD2B (rat): Y127‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y535-p - Src (mouse)
Modsite: FTSTEPQyQPGENL_ SwissProt Entrez-Gene
Orthologous residues
Src (human): Y530‑p, Src iso2 (human): Y536‑p, Src (mouse): Y535‑p, Src iso2 (mouse): Y529‑p, Src (rat): Y530‑p, Src (chicken): Y527‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y535-p - Yes (mouse)
Modsite: FTATEPQyQPGENL_ SwissProt Entrez-Gene
Orthologous residues
Yes (human): Y537‑p, Yes (mouse): Y535‑p, Yes (rat): Y535‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse