Curated Information
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Home > Curated Information Page > Cst Curation Set #147
Moritz A (2003) CST Curation Set: 147; Year: 2003; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y697-p - AXL (mouse)
Modsite: KIyNGDyyRQGRIAK SwissProt Entrez-Gene
Orthologous residues
AXL (human): Y703‑p, AXL iso2 (human): Y694‑p, AXL (mouse): Y697‑p, AXL (rat): Y697‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y889-p - Cbl-b (mouse)
Modsite: ANRAsQDyDQLPSss SwissProt Entrez-Gene
Orthologous residues
Cbl‑b (human): Y889‑p, Cbl‑b iso2 (human): , Cbl‑b (mouse): Y889‑p, Cbl‑b (rat): Y845‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y15-p - CDK1 (mouse)
Modsite: EKIGEGtyGVVyKGR SwissProt Entrez-Gene
Orthologous residues
CDK1 (human): Y15‑p, CDK1 iso2 (human): Y15‑p, CDK1 (mouse): Y15‑p, CDK1 (rat): Y15‑p, CDK1 (chicken): Y15‑p, CDK1 (fruit fly): Y15‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y15-p - CDK2 (mouse)
Modsite: EKIGEGtyGVVyKAK SwissProt Entrez-Gene
Orthologous residues
CDK2 (human): Y15‑p, CDK2 iso2 (human): Y15‑p, CDK2 (mouse): Y15‑p, CDK2 (rat): Y15‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y15-p - CDK3 (mouse)
Modsite: EKIGEGtyGVVyKAR SwissProt Entrez-Gene
Orthologous residues
CDK3 (human): Y15‑p, CDK3 (mouse): Y15‑p, CDK3 (cow): Y15‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y140-p - Cofilin-1 (mouse)
Modsite: HELQANCyEEVkDRC SwissProt Entrez-Gene
Orthologous residues
Cofilin‑1 (human): Y140‑p, Cofilin‑1 (mouse): Y140‑p, Cofilin‑1 (rat): Y140‑p, Cofilin‑1 (pig): Y140‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y471-p - DDR2 (mouse)
Modsite: EQESNSTyDRIFPLR SwissProt Entrez-Gene
Orthologous residues
DDR2 (human): Y471‑p, DDR2 (mouse): Y471‑p, DDR2 (rat): Y471‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y321-p - DYRK1A (mouse)
Modsite: LGQRIyQyIQsRFYR SwissProt Entrez-Gene
Orthologous residues
DYRK1A (human): Y321‑p, DYRK1A iso2 (human): Y312‑p, DYRK1A iso4 (human): Y321‑p, DYRK1A (mouse): Y321‑p, DYRK1A (rat): Y321‑p, DYRK1A (rabbit):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y273-p - DYRK1B (mouse)
Modsite: LGQRIyQyIQsRFYR SwissProt Entrez-Gene
Orthologous residues
DYRK1B (human): Y273‑p, DYRK1B iso2 (human): Y273‑p, DYRK1B (mouse): Y273‑p, DYRK1B (rat): Y273‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y59-p - DYRK1C (mouse)
Modsite: LGQRIyQyIQsRFYR SwissProt Entrez-Gene
Orthologous residues
DYRK1C (mouse): Y59‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y29-p - EEF1A1 (mouse)
Modsite: TTTGHLIykCGGIDk SwissProt Entrez-Gene
Orthologous residues
EEF1A1 (human): Y29‑p, EEF1A1 (mouse): Y29‑p, EEF1A1 (rat): Y29‑p, EEF1A1 (rabbit): Y29‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y29-p - EEF1A2 (mouse)
Modsite: TTTGHLIykCGGIDk SwissProt Entrez-Gene
Orthologous residues
EEF1A2 (human): Y29‑p, EEF1A2 (mouse): Y29‑p, EEF1A2 (rat): Y29‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y606-p - EPB41L2 (mouse)
Modsite: RVDGDNIyVRHsNLM SwissProt Entrez-Gene
Orthologous residues
EPB41L2 (human): Y623‑p, EPB41L2 iso2 (human): , EPB41L2 iso4 (human): , EPB41L2 iso6 (human): Y114‑p, EPB41L2 (mouse): Y606‑p, EPB41L2 iso3 (mouse): , EPB41L2 iso4 (mouse): , EPB41L2 (rat): Y615‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y479-p - EPB41L3 (mouse)
Modsite: AEVGtGQyATTKGIs SwissProt Entrez-Gene
Orthologous residues
EPB41L3 (human): Y453‑p, EPB41L3 iso2 (human): Y471‑p, EPB41L3 (mouse): Y479‑p, EPB41L3 iso7 (mouse): Y461‑p, EPB41L3 (rat): Y474‑p, EPB41L3 iso2 (rat): Y456‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y773-p - EphA2 (mouse)
Modsite: EDDPEAtyTtsGGKI SwissProt Entrez-Gene
Orthologous residues
EphA2 (human): Y772‑p, EphA2 (mouse): Y773‑p, EphA2 (rat): Y773‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y779-p - EphA3 (mouse)
Modsite: EDDPEAAyttRGGKI SwissProt Entrez-Gene
Orthologous residues
EphA3 (human): Y779‑p, EPHA3 iso2 (human): , EphA3 (mouse): Y779‑p, EphA3 (rat): Y780‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y779-p - EphA4 (mouse)
Modsite: EDDPEAAyttRGGKI SwissProt Entrez-Gene
Orthologous residues
EphA4 (human): Y779‑p, EphA4 (mouse): Y779‑p, EphA4 (rat): Y779‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y672-p - EphA5 (mouse)
Modsite: EDDPEAAyttRGGKI SwissProt Entrez-Gene
Orthologous residues
EphA5 (human): Y833‑p, EphA5 iso2 (human): Y811‑p, EphA5 iso3 (human): Y765‑p, EphA5 (mouse): Y672‑p, EphA5 (rat): Y835‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y181-p - epsin 2 (mouse)
Modsite: QPNLSTSysEQEyGk SwissProt Entrez-Gene
Orthologous residues
epsin 2 (human): H181‑p, epsin 2 iso2 (human): H181‑p, epsin 2 (mouse): Y181‑p, epsin 2 iso2 (mouse): Y181‑p, epsin 2 iso3 (mouse): Y181‑p, epsin 2 (rat): Y181‑p, epsin 2 iso1 (rat): H181‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y607-p - FAK iso2 (mouse)
Modsite: RyMEDstyyKAsKGK SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y576‑p, FAK iso2 (human): Y424‑p, FAK iso5 (human): Y576‑p, FAK (mouse): Y576‑p, FAK iso2 (mouse): Y607‑p, FAK iso4 (mouse): Y576‑p, FAK iso9 (mouse): , FAK (rat): Y576‑p, FAK (chicken): Y576‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y608-p - FAK iso2 (mouse)
Modsite: yMEDstyyKAsKGKL SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y577‑p, FAK iso2 (human): Y425‑p, FAK iso5 (human): Y577‑p, FAK (mouse): Y577‑p, FAK iso2 (mouse): Y608‑p, FAK iso4 (mouse): Y577‑p, FAK iso9 (mouse): , FAK (rat): Y577‑p, FAK (chicken): Y577‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y2502-p - FLNB (mouse)
Modsite: RSSTETCysAIPKSS SwissProt Entrez-Gene
Orthologous residues
FLNB (human): Y2502‑p, FLNB iso2 (human): Y2478‑p, FLNB iso8 (human): Y2533‑p, FLNB (mouse): Y2502‑p, FLNB (rat): Y2478‑p, FLNB (cow): Y2533‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y407-p - GAB1 (mouse)
Modsite: DASsQDCyDIPRtFP SwissProt Entrez-Gene
Orthologous residues
GAB1 (human): Y406‑p, GAB1 iso2 (human): Y406‑p, GAB1 (mouse): Y407‑p, GAB1 (rat): Y405‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y541-p - GIT2 (mouse)
Modsite: NstPEsDyDNTACDP SwissProt Entrez-Gene
Orthologous residues
GIT2 (human): Y592‑p, GIT2 iso5 (human): Y562‑p, GIT2 iso10 (human): Y514‑p, GIT2 (mouse): Y541‑p, GIT2 iso3 (mouse): Y512‑p, GIT2 iso4 (mouse): Y592‑p, GIT2 iso5 (mouse): Y514‑p, GIT2 (rat): Y592‑p, GIT2 (chicken): Y592‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y279-p - GSK3A (mouse)
Modsite: RGEPNVsyICsRYYR SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): Y279‑p, GSK3A (mouse): Y279‑p, GSK3A (rat): Y279‑p, GSK3A (cow): Y279‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y216-p - GSK3B (mouse)
Modsite: RGEPNVsyICsRYYR SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): Y216‑p, GSK3B iso2 (human): Y216‑p, GSK3B (mouse): Y216‑p, GSK3B (rat): Y216‑p, GSK3B (rabbit): Y216‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y409-p - Hck (mouse)
Modsite: RIIEDNEytAREGAK SwissProt Entrez-Gene
Orthologous residues
Hck (human): Y411‑p, Hck iso2 (human): Y390‑p, Hck iso3 (human): Y389‑p, Hck iso4 (human): Y410‑p, Hck (mouse): Y409‑p, Hck (rat): Y409‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y352-p - HIPK1 (mouse)
Modsite: SKAVCStyLQsRYYR SwissProt Entrez-Gene
Orthologous residues
HIPK1 (human): Y352‑p, HIPK1 iso2 (human): Y352‑p, HIPK1 iso4 (human): , HIPK1 (mouse): Y352‑p, HIPK1 (rat): Y352‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y361-p - HIPK2 (mouse)
Modsite: SKAVCStyLQsRYYR SwissProt Entrez-Gene
Orthologous residues
HIPK2 (human): Y361‑p, HIPK2 (mouse): Y361‑p, HIPK2 (rat): Y361‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y359-p - HIPK3 (mouse)
Modsite: SKTVCStyLQsRYYR SwissProt Entrez-Gene
Orthologous residues
HIPK3 (human): Y359‑p, HIPK3 iso2 (human): Y359‑p, HIPK3 (mouse): Y359‑p, HIPK3 (rat): Y359‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y182-p - P38A (mouse)
Modsite: tDDEMtGyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y118-p - PXN (mouse)
Modsite: AGEEEHVysFPNKQk SwissProt Entrez-Gene
Orthologous residues
PXN (human): Y118‑p, PXN iso2 (human): Y118‑p, PXN iso3 (human): Y118‑p, PXN (mouse): Y118‑p, PXN iso2 (mouse): Y118‑p, PXN (rat): Y118‑p, PXN (chicken): Y118‑p, PXN (cow): Y112‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y391-p - SGK223 (mouse)
Modsite: PVQPEPIyAEsAKRK SwissProt Entrez-Gene
Orthologous residues
SGK223 (human): Y413‑p, SGK223 (mouse): Y391‑p, SGK223 (rat): Y391‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y113-p - SHB (mouse)
Modsite: RAMCRLDyCGGGGGG SwissProt Entrez-Gene
Orthologous residues
SHB (human): Y114‑p, SHB iso2 (human): Y114‑p, SHB (mouse): Y113‑p, SHB (rat): Y113‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y84-p - SMYD4 (mouse)
Modsite: RKFQEKEyTDAAVLy SwissProt Entrez-Gene
Orthologous residues
SMYD4 (human): Y84‑p, SMYD4 (mouse): Y84‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y91-p - SMYD4 (mouse)
Modsite: yTDAAVLySKGVSHS SwissProt Entrez-Gene
Orthologous residues
SMYD4 (human): Y91‑p, SMYD4 (mouse): Y91‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y705-p - STAT3 (mouse)
Modsite: DPGsAAPyLktkFIC SwissProt Entrez-Gene
Orthologous residues
STAT3 (human): Y705‑p, STAT3 iso2 (human): Y704‑p, STAT3 iso3 (human): Y705‑p, STAT3 (mouse): Y705‑p, STAT3 iso2 (mouse): Y705‑p, STAT3 iso3 (mouse): Y704‑p, STAT3 (rat): Y705‑p, STAT3 (cow): Y705‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y1477-p - tensin 1 (mouse)
Modsite: sLDRHAAyGGystPE SwissProt Entrez-Gene
Orthologous residues
tensin 1 (human): Y1323‑p, tensin 1 iso2 (human): , tensin 1 (mouse): Y1477‑p, tensin 1 (rat): Y1496‑p, tensin 1 (cow): Y1302‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y1480-p - tensin 1 (mouse)
Modsite: RHAAyGGystPEDRR SwissProt Entrez-Gene
Orthologous residues
tensin 1 (human): Y1326‑p, tensin 1 iso2 (human): , tensin 1 (mouse): Y1480‑p, tensin 1 (rat): Y1499‑p, tensin 1 (cow): Y1305‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y1558-p - tensin 1 (mouse)
Modsite: RAGsLPNyAtINGKV SwissProt Entrez-Gene
Orthologous residues
tensin 1 (human): Y1404‑p, tensin 1 iso2 (human): , tensin 1 (mouse): Y1558‑p, tensin 1 (rat): Y1577‑p, tensin 1 (cow): Y1383‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y483-p - tensin 2 (mouse)
Modsite: GPLDGsPyAQVQRVP SwissProt Entrez-Gene
Orthologous residues
tensin 2 (human): Y483‑p, tensin 2 iso2 (human): Y483‑p, tensin 2 iso4 (human): Y493‑p, tensin 2 (mouse): Y483‑p, tensin 2 (rat): Y483‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y160-p - YB-1 (mouse)
Modsite: PRNyQQNyQNsEsGE SwissProt Entrez-Gene
Orthologous residues
YB‑1 (human): Y162‑p, YB‑1 (mouse): Y160‑p, YB‑1 (rat): Y160‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y715-p - ZCCHC11 (mouse)
Modsite: NSQLVYEyVVERFRA SwissProt Entrez-Gene
Orthologous residues
ZCCHC11 (human): Y695‑p, ZCCHC11 iso2 (human): , ZCCHC11 (mouse): Y715‑p, ZCCHC11 (rat): Y559‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y1164-p - ZO1 (mouse)
Modsite: EEQPAPAyEVHNRYR SwissProt Entrez-Gene
Orthologous residues
ZO1 (human): Y1165‑p, ZO1 iso2 (human): Y1085‑p, ZO1 (mouse): Y1164‑p, ZO1 iso2 (mouse): Y1084‑p, ZO1 (rat): Y1152‑p, ZO1 (cow): Y1126‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse