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Home > Curated Information Page > PubMed Id: 19638400
Figueroa-Romero C, et al. (2009) SUMOylation of the mitochondrial fission protein Drp1 occurs at multiple nonconsensus sites within the B domain and is linked to its activity cycle. FASEB J 23, 3917-27 19638400
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K532-sm - DRP1 (human)
Modsite: PSAVsRDkSSkVPSA SwissProt Entrez-Gene
Orthologous residues
DRP1 (human): K532‑sm, DRP1 iso2 (human): K532‑sm, DRP1 iso3 (human): K532‑sm, DRP1 iso4 (human): K532‑sm, DRP1 iso6 (human): K545‑sm, DRP1 (mouse): K532‑sm, DRP1 iso3 (mouse): K532‑sm, DRP1 iso6 (mouse): K545‑sm, DRP1 (rat): K545‑sm
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
SUMO LIGASE UBC9 (human) transfection of wild-type enzyme, two-hybrid system
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Comments:  sumoylation is linked to its GTPase activity cycle, and influenced by DRP1 localization

K535-sm - DRP1 (human)
Modsite: VsRDkSSkVPSALAP SwissProt Entrez-Gene
Orthologous residues
DRP1 (human): K535‑sm, DRP1 iso2 (human): K535‑sm, DRP1 iso3 (human): , DRP1 iso4 (human): , DRP1 iso6 (human): K548‑sm, DRP1 (mouse): K535‑sm, DRP1 iso3 (mouse): , DRP1 iso6 (mouse): , DRP1 (rat): K548‑sm
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
SUMO LIGASE UBC9 (human) transfection of wild-type enzyme, two-hybrid system
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Comments:  sumoylation is linked to its GTPase activity cycle, and influenced by DRP1 localization

K558-sm - DRP1 (human)
Modsite: AsAEADGkLIQDSRR SwissProt Entrez-Gene
Orthologous residues
DRP1 (human): K558‑sm, DRP1 iso2 (human): K558‑sm, DRP1 iso3 (human): , DRP1 iso4 (human): , DRP1 iso6 (human): K571‑sm, DRP1 (mouse): K558‑sm, DRP1 iso3 (mouse): , DRP1 iso6 (mouse): , DRP1 (rat): K571‑sm
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
SUMO LIGASE UBC9 (human) transfection of wild-type enzyme, two-hybrid system
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Comments:  sumoylation is linked to its GTPase activity cycle, and influenced by DRP1 localization

K568-sm - DRP1 (human)
Modsite: QDSRRETkNVAsGGG SwissProt Entrez-Gene
Orthologous residues
DRP1 (human): K568‑sm, DRP1 iso2 (human): , DRP1 iso3 (human): K542‑sm, DRP1 iso4 (human): , DRP1 iso6 (human): K581‑sm, DRP1 (mouse): K568‑sm, DRP1 iso3 (mouse): , DRP1 iso6 (mouse): , DRP1 (rat): K581‑sm
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
SUMO LIGASE UBC9 (human) transfection of wild-type enzyme, two-hybrid system
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Comments:  sumoylation is linked to its GTPase activity cycle, and influenced by DRP1 localization

K594-sm - DRP1 (human)
Modsite: GNWRGMLktSkAEEL SwissProt Entrez-Gene
Orthologous residues
DRP1 (human): K594‑sm, DRP1 iso2 (human): K583‑sm, DRP1 iso3 (human): K568‑sm, DRP1 iso4 (human): K557‑sm, DRP1 iso6 (human): K607‑sm, DRP1 (mouse): K594‑sm, DRP1 iso3 (mouse): K557‑sm, DRP1 iso6 (mouse): K570‑sm, DRP1 (rat): K613‑sm
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
SUMO LIGASE UBC9 (human) transfection of wild-type enzyme, two-hybrid system
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Comments:  sumoylation is linked to its GTPase activity cycle, and influenced by DRP1 localization

K597-sm - DRP1 (human)
Modsite: RGMLktSkAEELLAE SwissProt Entrez-Gene
Orthologous residues
DRP1 (human): K597‑sm, DRP1 iso2 (human): K586‑sm, DRP1 iso3 (human): K571‑sm, DRP1 iso4 (human): K560‑sm, DRP1 iso6 (human): K610‑sm, DRP1 (mouse): K597‑sm, DRP1 iso3 (mouse): K560‑sm, DRP1 iso6 (mouse): K573‑sm, DRP1 (rat): K616‑sm
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
SUMO LIGASE UBC9 (human) transfection of wild-type enzyme, two-hybrid system
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Comments:  sumoylation is linked to its GTPase activity cycle, and influenced by DRP1 localization

K606-sm - DRP1 (human)
Modsite: EELLAEEkskPIPIM SwissProt Entrez-Gene
Orthologous residues
DRP1 (human): K606‑sm, DRP1 iso2 (human): K595‑sm, DRP1 iso3 (human): K580‑sm, DRP1 iso4 (human): K569‑sm, DRP1 iso6 (human): K619‑sm, DRP1 (mouse): K606‑sm, DRP1 iso3 (mouse): K569‑sm, DRP1 iso6 (mouse): K582‑sm, DRP1 (rat): K625‑sm
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
SUMO LIGASE UBC9 (human) transfection of wild-type enzyme, two-hybrid system
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Comments:  sumoylation is linked to its GTPase activity cycle, and influenced by DRP1 localization

K608-sm - DRP1 (human)
Modsite: LLAEEkskPIPIMPA SwissProt Entrez-Gene
Orthologous residues
DRP1 (human): K608‑sm, DRP1 iso2 (human): K597‑sm, DRP1 iso3 (human): K582‑sm, DRP1 iso4 (human): K571‑sm, DRP1 iso6 (human): K621‑sm, DRP1 (mouse): K608‑sm, DRP1 iso3 (mouse): K571‑sm, DRP1 iso6 (mouse): K584‑sm, DRP1 (rat): K627‑sm
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
SUMO LIGASE UBC9 (human) transfection of wild-type enzyme, two-hybrid system
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Comments:  sumoylation is linked to its GTPase activity cycle, and influenced by DRP1 localization