Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.4
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 17425515
Costantini C, Ko MH, Jonas MC, Puglielli L (2007) A reversible form of lysine acetylation in the ER and Golgi lumen controls the molecular stabilization of BACE1. Biochem J 407, 383-95 17425515
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

K126-ac - BACE (human)
Modsite: STYRDLRkGVYVPYT SwissProt Entrez-Gene
Orthologous residues
BACE (human): K126‑ac, BACE (mouse): K126‑ac, BACE (rat): K126‑ac
Characterization
Methods used to characterize site in vivo [14C] acetate labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  CHO (fibroblast), SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
ACETYLTRANSFERASE CBP (human)
Downstream Regulation
Effect of modification (function):  intracellular localization, protein stabilization
Comments:  regulates translocation through secretory pathway

K275-ac - BACE (human)
Modsite: EINGQDLkMDCkEYN SwissProt Entrez-Gene
Orthologous residues
BACE (human): K275‑ac, BACE (mouse): K275‑ac, BACE (rat): K275‑ac
Characterization
Methods used to characterize site in vivo [14C] acetate labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  CHO (fibroblast), SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
ACETYLTRANSFERASE CBP (human)
Downstream Regulation
Effect of modification (function):  intracellular localization, protein stabilization
Comments:  regulates translocation through secretory pathway

K279-ac - BACE (human)
Modsite: QDLkMDCkEYNYDkS SwissProt Entrez-Gene
Orthologous residues
BACE (human): K279‑ac, BACE (mouse): K279‑ac, BACE (rat): K279‑ac
Characterization
Methods used to characterize site in vivo [14C] acetate labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  CHO (fibroblast), SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
ACETYLTRANSFERASE CBP (human)
Downstream Regulation
Effect of modification (function):  intracellular localization, protein stabilization
Comments:  regulates translocation through secretory pathway

K285-ac - BACE (human)
Modsite: CkEYNYDkSIVDSGT SwissProt Entrez-Gene
Orthologous residues
BACE (human): K285‑ac, BACE (mouse): K285‑ac, BACE (rat): K285‑ac
Characterization
Methods used to characterize site in vivo [14C] acetate labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  CHO (fibroblast), SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
ACETYLTRANSFERASE CBP (human)
Downstream Regulation
Effect of modification (function):  intracellular localization, protein stabilization
Comments:  regulates translocation through secretory pathway

K299-ac - BACE (human)
Modsite: TTNLRLPkkVFEAAV SwissProt Entrez-Gene
Orthologous residues
BACE (human): K299‑ac, BACE (mouse): K299‑ac, BACE (rat): K299‑ac
Characterization
Methods used to characterize site in vivo [14C] acetate labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  CHO (fibroblast), SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE CBP (human)
ACETYLTRANSFERASE p300 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization, protein stabilization
Comments:  regulates translocation through secretory pathway

K300-ac - BACE (human)
Modsite: TNLRLPkkVFEAAVk SwissProt Entrez-Gene
Orthologous residues
BACE (human): K300‑ac, BACE (mouse): K300‑ac, BACE (rat): K300‑ac
Characterization
Methods used to characterize site in vivo [14C] acetate labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  CHO (fibroblast), SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE CBP (human)
ACETYLTRANSFERASE p300 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization, protein stabilization
Comments:  regulates translocation through secretory pathway

K307-ac - BACE (human)
Modsite: kVFEAAVksIKAASS SwissProt Entrez-Gene
Orthologous residues
BACE (human): K307‑ac, BACE (mouse): K307‑ac, BACE (rat): K307‑ac
Characterization
Methods used to characterize site in vivo [14C] acetate labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  CHO (fibroblast), SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
ACETYLTRANSFERASE CBP (human)
Downstream Regulation
Effect of modification (function):  intracellular localization, protein stabilization
Comments:  regulates translocation through secretory pathway