Costantini C, Ko MH, Jonas MC, Puglielli L (2007) A reversible form of lysine acetylation in the ER and Golgi lumen controls the molecular stabilization of BACE1. Biochem J 407, 383-95 17425515

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K126-ac - BACE (human) ▲

Modsite: STYRDLRkGVYVPYT SwissProt Entrez-Gene Orthologous residues BACE (human): K126‑ac, BACE (mouse): K126‑ac, BACE (rat): K126‑ac Characterization Methods used to characterize site in vivo:  [14C] acetate labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  CHO (fibroblast), SH-SY5Y (neural crest) Cellular systems studied:  cell lines Species studied:  hamster, human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) ACETYLTRANSFERASE CBP (human) Downstream Regulation Effect of modification (function):  intracellular localization, protein stabilization Comments:  regulates translocation through secretory pathway

K275-ac - BACE (human) ▲

Modsite: EINGQDLkMDCkEYN SwissProt Entrez-Gene Orthologous residues BACE (human): K275‑ac, BACE (mouse): K275‑ac, BACE (rat): K275‑ac Characterization Methods used to characterize site in vivo:  [14C] acetate labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  CHO (fibroblast), SH-SY5Y (neural crest) Cellular systems studied:  cell lines Species studied:  hamster, human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) ACETYLTRANSFERASE CBP (human) Downstream Regulation Effect of modification (function):  intracellular localization, protein stabilization Comments:  regulates translocation through secretory pathway

K279-ac - BACE (human) ▲

Modsite: QDLkMDCkEYNYDkS SwissProt Entrez-Gene Orthologous residues BACE (human): K279‑ac, BACE (mouse): K279‑ac, BACE (rat): K279‑ac Characterization Methods used to characterize site in vivo:  [14C] acetate labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  CHO (fibroblast), SH-SY5Y (neural crest) Cellular systems studied:  cell lines Species studied:  hamster, human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) ACETYLTRANSFERASE CBP (human) Downstream Regulation Effect of modification (function):  intracellular localization, protein stabilization Comments:  regulates translocation through secretory pathway

K285-ac - BACE (human) ▲

Modsite: CkEYNYDkSIVDSGT SwissProt Entrez-Gene Orthologous residues BACE (human): K285‑ac, BACE (mouse): K285‑ac, BACE (rat): K285‑ac Characterization Methods used to characterize site in vivo:  [14C] acetate labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  CHO (fibroblast), SH-SY5Y (neural crest) Cellular systems studied:  cell lines Species studied:  hamster, human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) ACETYLTRANSFERASE CBP (human) Downstream Regulation Effect of modification (function):  intracellular localization, protein stabilization Comments:  regulates translocation through secretory pathway

K299-ac - BACE (human) ▲

Modsite: TTNLRLPkkVFEAAV SwissProt Entrez-Gene Orthologous residues BACE (human): K299‑ac, BACE (mouse): K299‑ac, BACE (rat): K299‑ac Characterization Methods used to characterize site in vivo:  [14C] acetate labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  CHO (fibroblast), SH-SY5Y (neural crest) Cellular systems studied:  cell lines Species studied:  hamster, human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE CBP (human) ACETYLTRANSFERASE p300 (human) Downstream Regulation Effect of modification (function):  intracellular localization, protein stabilization Comments:  regulates translocation through secretory pathway

K300-ac - BACE (human) ▲

Modsite: TNLRLPkkVFEAAVk SwissProt Entrez-Gene Orthologous residues BACE (human): K300‑ac, BACE (mouse): K300‑ac, BACE (rat): K300‑ac Characterization Methods used to characterize site in vivo:  [14C] acetate labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  CHO (fibroblast), SH-SY5Y (neural crest) Cellular systems studied:  cell lines Species studied:  hamster, human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE CBP (human) ACETYLTRANSFERASE p300 (human) Downstream Regulation Effect of modification (function):  intracellular localization, protein stabilization Comments:  regulates translocation through secretory pathway

K307-ac - BACE (human) ▲

Modsite: kVFEAAVksIKAASS SwissProt Entrez-Gene Orthologous residues BACE (human): K307‑ac, BACE (mouse): K307‑ac, BACE (rat): K307‑ac Characterization Methods used to characterize site in vivo:  [14C] acetate labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  CHO (fibroblast), SH-SY5Y (neural crest) Cellular systems studied:  cell lines Species studied:  hamster, human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) ACETYLTRANSFERASE CBP (human) Downstream Regulation Effect of modification (function):  intracellular localization, protein stabilization Comments:  regulates translocation through secretory pathway