Moritz A (2003) CST Curation Set: 91; Year: 2003; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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Y326-p - CD34 (mouse) ▲
Modsite: ERLGEDPyyTENGGG SwissProt Entrez-Gene Orthologous residues CD34 (human): Y329‑p, CD34 iso2 (human): ‑, CD34 (mouse): Y326‑p, CD34 iso2 (mouse): ‑, CD34 (rat): Y330‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y213-p - CDV-3 (mouse) ▲
Modsite: PQGPPEIysDTQFPs SwissProt Entrez-Gene Orthologous residues CDV‑3 (human): Y190‑p, CDV‑3 iso2 (human): Y190‑p, CDV‑3 (mouse): Y213‑p, CDV‑3 (rat): Y213‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y734-p - CENTB2 (mouse) ▲
Modsite: MREsEGLyGQPGDET SwissProt Entrez-Gene Orthologous residues CENTB2 (human): Y742‑p, CENTB2 (mouse): Y734‑p, CENTB2 (rat): Y734‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y140-p - Cofilin-1 (mouse) ▲
Modsite: HELQANCyEEVkDRC SwissProt Entrez-Gene Orthologous residues Cofilin‑1 (human): Y140‑p, Cofilin‑1 (mouse): Y140‑p, Cofilin‑1 (rat): Y140‑p, Cofilin‑1 (pig): Y140‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y228-p - CTNND1 (mouse) ▲

Modsite: yPGGsDNyGsLsRVT SwissProt Entrez-Gene Orthologous residues CTNND1 (human): Y228‑p, CTNND1 iso2 (human): Y228‑p, CTNND1 iso3 (human): Y228‑p, CTNND1 iso4 (human): Y228‑p, CTNND1 iso10 (human): Y174‑p, CTNND1 iso12 (human): Y174‑p, CTNND1 iso22 (human): Y127‑p, CTNND1 (mouse): Y228‑p, CTNND1 iso3 (mouse): Y228‑p, CTNND1 (rat): Y228‑p, CTNND1 (cow): Y228‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y342-p - DAB2 (mouse) ▲
Modsite: PLNGDtDyFGQQFDQ SwissProt Entrez-Gene Orthologous residues DAB2 (human): Y342‑p, DAB2 (mouse): Y342‑p, DAB2 (rat): Y342‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y314-p - DOK1 (mouse) ▲
Modsite: GPSQDSVysDPLGST SwissProt Entrez-Gene Orthologous residues DOK1 (human): Y315‑p, DOK1 iso3 (human): Y176‑p, DOK1 (mouse): Y314‑p, DOK1 (rat): Y314‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y361-p - DOK1 (mouse) ▲
Modsite: DsKEDPIyDEPEGLA SwissProt Entrez-Gene Orthologous residues DOK1 (human): Y362‑p, DOK1 iso3 (human): Y223‑p, DOK1 (mouse): Y361‑p, DOK1 (rat): Y361‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y450-p - DOK1 (mouse) ▲
Modsite: FSSDTALySQVQKSG SwissProt Entrez-Gene Orthologous residues DOK1 (human): Y449‑p, DOK1 iso3 (human): Y310‑p, DOK1 (mouse): Y450‑p, DOK1 (rat): Y448‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y315-p - endophilin A2 (mouse) ▲
Modsite: QPSCKALyDFEPEND SwissProt Entrez-Gene Orthologous residues endophilin A2 (human): Y315‑p, endophilin A2 iso2 (human): Y267‑p, endophilin A2 (mouse): Y315‑p, endophilin A2 (rat): Y315‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y25-p - ENO1 (mouse) ▲
Modsite: PTVEVDLytAkGLFR SwissProt Entrez-Gene Orthologous residues ENO1 (human): F25‑p, ENO1 iso2 (human): ‑, ENO1 (mouse): Y25‑p, ENO1 (rat): Y25‑p, ENO1 (chicken): Y25‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y44-p - ENO1 (mouse) ▲
Modsite: sGAstGIyEALELRD SwissProt Entrez-Gene Orthologous residues ENO1 (human): Y44‑p, ENO1 iso2 (human): ‑, ENO1 (mouse): Y44‑p, ENO1 (rat): Y44‑p, ENO1 (chicken): Y44‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y25-p - ENO2 (mouse) ▲
Modsite: PTVEVDLytAkGLFR SwissProt Entrez-Gene Orthologous residues ENO2 (human): Y25‑p, ENO2 (mouse): Y25‑p, ENO2 (rat): H25‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y44-p - ENO2 (mouse) ▲
Modsite: sGAstGIyEALELRD SwissProt Entrez-Gene Orthologous residues ENO2 (human): Y44‑p, ENO2 (mouse): Y44‑p, ENO2 (rat): Y44‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y44-p - ENO3 (mouse) ▲
Modsite: sGAstGIyEALELRD SwissProt Entrez-Gene Orthologous residues ENO3 (human): Y44‑p, ENO3 iso100 (human): Y44‑p, ENO3 (mouse): Y44‑p, ENO3 (rat): Y44‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y690-p - EPRS (mouse) ▲
Modsite: PYEPVsPysCREAPC SwissProt Entrez-Gene Orthologous residues EPRS (human): Y690‑p, EPRS (mouse): Y690‑p, EPRS (rat): Y664‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y316-p - GAPDH (mouse) ▲
Modsite: ISWYDNEyGySNRVV SwissProt Entrez-Gene Orthologous residues GAPDH (human): F318‑p, GAPDH iso2 (human): F276‑p, GAPDH (mouse): Y316‑p, GAPDH (rat): Y316‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y301-p - LPP (mouse) ▲
Modsite: QGRyyEPyyAAGPSy SwissProt Entrez-Gene Orthologous residues LPP (human): Y300‑p, LPP (mouse): Y301‑p, LPP (rat): F297‑p, LPP (cow): Y302‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y302-p - LPP (mouse) ▲
Modsite: GRyyEPyyAAGPSyG SwissProt Entrez-Gene Orthologous residues LPP (human): Y301‑p, LPP (mouse): Y302‑p, LPP (rat): F298‑p, LPP (cow): C303‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y132-p - P130Cas (mouse) ▲

Modsite: SKTQQGLyQAPGPNP SwissProt Entrez-Gene Orthologous residues P130Cas (human): Y128‑p, P130Cas iso2 (human): Y146‑p, P130Cas iso6 (human): Y174‑p, P130Cas iso7 (human): Y146‑p, P130Cas iso8 (human): Y128‑p, P130Cas (mouse): Y132‑p, P130Cas (rat): Y226‑p, P130Cas iso2 (rat): Y132‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y226-p - P130Cas (mouse) ▲

Modsite: PTRVGQGyVyEAAQT SwissProt Entrez-Gene Orthologous residues P130Cas (human): Y222‑p, P130Cas iso2 (human): Y240‑p, P130Cas iso6 (human): Y268‑p, P130Cas iso7 (human): Y240‑p, P130Cas iso8 (human): Y222‑p, P130Cas (mouse): Y226‑p, P130Cas (rat): Y320‑p, P130Cas iso2 (rat): Y226‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y238-p - P130Cas (mouse) ▲

Modsite: AQTEQDEyDtPRHLL SwissProt Entrez-Gene Orthologous residues P130Cas (human): Y234‑p, P130Cas iso2 (human): Y252‑p, P130Cas iso6 (human): Y280‑p, P130Cas iso7 (human): Y252‑p, P130Cas iso8 (human): Y234‑p, P130Cas (mouse): Y238‑p, P130Cas (rat): Y332‑p, P130Cas iso2 (rat): Y238‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y632-p - PEAK1 (mouse) ▲
Modsite: IVINPNAyDNLAIyK SwissProt Entrez-Gene Orthologous residues PEAK1 (human): Y635‑p, PEAK1 (mouse): Y632‑p, PEAK1 (rat): Y631‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y638-p - PEAK1 (mouse) ▲
Modsite: AyDNLAIyKSFLGts SwissProt Entrez-Gene Orthologous residues PEAK1 (human): Y641‑p, PEAK1 (mouse): Y638‑p, PEAK1 (rat): Y637‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y607-p - PIK3R1 (mouse) ▲

Modsite: NENTEDQysLVEDDE SwissProt Entrez-Gene Orthologous residues PIK3R1 (human): Y607‑p, PIK3R1 iso2 (human): Y337‑p, PIK3R1 iso4 (human): Y615‑p, PIK3R1 (mouse): Y607‑p, PIK3R1 (rat): Y607‑p, PIK3R1 (cow): Y607‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y4622-p - Plectin-1 (mouse) ▲

Modsite: SkGyysPysVsGsGs SwissProt Entrez-Gene Orthologous residues Plectin‑1 (human): Y4615‑p, Plectin‑1 iso2 (human): Y4505‑p, Plectin‑1 iso3 (human): Y4501‑p, Plectin‑1 iso4 (human): Y4478‑p, Plectin‑1 iso5 (human): Y4478‑p, Plectin‑1 iso6 (human): Y4482‑p, Plectin‑1 iso8 (human): Y4456‑p, Plectin‑1 iso9 (human): Y4464‑p, Plectin‑1 (mouse): Y4622‑p, Plectin‑1 iso3 (mouse): Y4474‑p, Plectin‑1 iso6 (mouse): Y4503‑p, Plectin‑1 (rat): Y4618‑p, Plectin‑1 iso2 (rat): Y4475‑p, Plectin‑1 (cow): Y4575‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y310-p - PTRF (mouse) ▲

Modsite: FtPDHVVyARSKtAV SwissProt Entrez-Gene Orthologous residues PTRF (human): Y308‑p, PTRF iso2 (human): Y218‑p, PTRF (mouse): Y310‑p, PTRF iso3 (mouse): ‑, PTRF (rat): Y310‑p, PTRF (cow): Y308‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y118-p - PXN (mouse) ▲

Modsite: AGEEEHVysFPNKQk SwissProt Entrez-Gene Orthologous residues PXN (human): Y118‑p, PXN iso2 (human): Y118‑p, PXN iso3 (human): Y118‑p, PXN (mouse): Y118‑p, PXN iso2 (mouse): Y118‑p, PXN (rat): Y118‑p, PXN (chicken): Y118‑p, PXN (cow): Y112‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y17-p - SEPT2 (mouse) ▲
Modsite: INPEtPGyVGFANLP SwissProt Entrez-Gene Orthologous residues SEPT2 (human): Y17‑p, SEPT2 iso2 (human): Y52‑p, SEPT2 iso3 (human): Y17‑p, SEPT2 (mouse): Y17‑p, SEPT2 (rat): Y17‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y391-p - SGK223 (mouse) ▲
Modsite: PVQPEPIyAEsAKRK SwissProt Entrez-Gene Orthologous residues SGK223 (human): Y413‑p, SGK223 (mouse): Y391‑p, SGK223 (rat): Y391‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y423-p - SHC1 (mouse) ▲

Modsite: ELFDDPsyVNIQNLD SwissProt Entrez-Gene Orthologous residues SHC1 (human): Y427‑p, SHC1 iso2 (human): Y317‑p, SHC1 iso3 (human): Y272‑p, SHC1 iso6 (human): Y428‑p, SHC1 iso7 (human): Y318‑p, SHC1 (mouse): Y423‑p, SHC1 iso2 (mouse): Y313‑p, SHC1 iso3 (mouse): Y268‑p, SHC1 (rat): Y423‑p, SHC1 iso2 (rat): Y313‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y62-p - SHP-2 (mouse) ▲
Modsite: KIQNTGDyyDLyGGE SwissProt Entrez-Gene Orthologous residues SHP‑2 (human): Y62‑p, SHP‑2 iso1 (human): Y62‑p, SHP‑2 (mouse): Y62‑p, SHP‑2 (rat): Y62‑p, SHP‑2 iso1 (rat): Y62‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y260-p - SKAP2 (mouse) ▲
Modsite: QPIDDEIyEELPEEE SwissProt Entrez-Gene Orthologous residues SKAP2 (human): Y261‑p, SKAP2 (mouse): Y260‑p, SKAP2 (rat): Y260‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y805-p - VCP (mouse) ▲
Modsite: EDNDDDLyG______ SwissProt Entrez-Gene Orthologous residues VCP (human): Y805‑p, VCP (mouse): Y805‑p, VCP (rat): Y805‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y53-p - Vimentin (mouse) ▲

Modsite: PstsRsLysssPGGA SwissProt Entrez-Gene Orthologous residues Vimentin (human): Y53‑p, Vimentin (mouse): Y53‑p, Vimentin (rat): Y53‑p, Vimentin (rabbit): F53‑p, Vimentin (hamster): Y52‑p, Vimentin (cow): Y53‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse