Curated Information
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Home > Curated Information Page > Cst Curation Set #87
Moritz A (2003) CST Curation Set: 87; Year: 2003; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y321-p - DYRK1A (mouse)
Modsite: LGQRIyQyIQsRFYR SwissProt Entrez-Gene
Orthologous residues
DYRK1A (human): Y321‑p, DYRK1A iso2 (human): Y312‑p, DYRK1A iso4 (human): Y321‑p, DYRK1A (mouse): Y321‑p, DYRK1A (rat): Y321‑p, DYRK1A (rabbit):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y273-p - DYRK1B (mouse)
Modsite: LGQRIyQyIQsRFYR SwissProt Entrez-Gene
Orthologous residues
DYRK1B (human): Y273‑p, DYRK1B iso2 (human): Y273‑p, DYRK1B (mouse): Y273‑p, DYRK1B (rat): Y273‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y59-p - DYRK1C (mouse)
Modsite: LGQRIyQyIQsRFYR SwissProt Entrez-Gene
Orthologous residues
DYRK1C (mouse): Y59‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y29-p - EEF1A1 (mouse)
Modsite: TTTGHLIykCGGIDk SwissProt Entrez-Gene
Orthologous residues
EEF1A1 (human): Y29‑p, EEF1A1 (mouse): Y29‑p, EEF1A1 (rat): Y29‑p, EEF1A1 (rabbit): Y29‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y29-p - EEF1A2 (mouse)
Modsite: TTTGHLIykCGGIDk SwissProt Entrez-Gene
Orthologous residues
EEF1A2 (human): Y29‑p, EEF1A2 (mouse): Y29‑p, EEF1A2 (rat): Y29‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y773-p - EphA2 (mouse)
Modsite: EDDPEAtyTtsGGKI SwissProt Entrez-Gene
Orthologous residues
EphA2 (human): Y772‑p, EphA2 (mouse): Y773‑p, EphA2 (rat): Y773‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y779-p - EphA3 (mouse)
Modsite: EDDPEAAyttRGGKI SwissProt Entrez-Gene
Orthologous residues
EphA3 (human): Y779‑p, EPHA3 iso2 (human): , EphA3 (mouse): Y779‑p, EphA3 (rat): Y780‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y779-p - EphA4 (mouse)
Modsite: EDDPEAAyttRGGKI SwissProt Entrez-Gene
Orthologous residues
EphA4 (human): Y779‑p, EphA4 (mouse): Y779‑p, EphA4 (rat): Y779‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y672-p - EphA5 (mouse)
Modsite: EDDPEAAyttRGGKI SwissProt Entrez-Gene
Orthologous residues
EphA5 (human): Y833‑p, EphA5 iso2 (human): Y811‑p, EphA5 iso3 (human): Y765‑p, EphA5 (mouse): Y672‑p, EphA5 (rat): Y835‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y607-p - FAK iso2 (mouse)
Modsite: RyMEDstyyKAsKGK SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y576‑p, FAK iso2 (human): Y424‑p, FAK iso5 (human): Y576‑p, FAK (mouse): Y576‑p, FAK iso2 (mouse): Y607‑p, FAK iso4 (mouse): Y576‑p, FAK iso9 (mouse): , FAK (rat): Y576‑p, FAK (chicken): Y576‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y541-p - GIT2 (mouse)
Modsite: NstPEsDyDNTACDP SwissProt Entrez-Gene
Orthologous residues
GIT2 (human): Y592‑p, GIT2 iso5 (human): Y562‑p, GIT2 iso10 (human): Y514‑p, GIT2 (mouse): Y541‑p, GIT2 iso3 (mouse): Y512‑p, GIT2 iso4 (mouse): Y592‑p, GIT2 iso5 (mouse): Y514‑p, GIT2 (rat): Y592‑p, GIT2 (chicken): Y592‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y352-p - HIPK1 (mouse)
Modsite: SKAVCStyLQsRYYR SwissProt Entrez-Gene
Orthologous residues
HIPK1 (human): Y352‑p, HIPK1 iso2 (human): Y352‑p, HIPK1 iso4 (human): , HIPK1 (mouse): Y352‑p, HIPK1 (rat): Y352‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y361-p - HIPK2 (mouse)
Modsite: SKAVCStyLQsRYYR SwissProt Entrez-Gene
Orthologous residues
HIPK2 (human): Y361‑p, HIPK2 (mouse): Y361‑p, HIPK2 (rat): Y361‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y118-p - PXN (mouse)
Modsite: AGEEEHVysFPNKQk SwissProt Entrez-Gene
Orthologous residues
PXN (human): Y118‑p, PXN iso2 (human): Y118‑p, PXN iso3 (human): Y118‑p, PXN (mouse): Y118‑p, PXN iso2 (mouse): Y118‑p, PXN (rat): Y118‑p, PXN (chicken): Y118‑p, PXN (cow): Y112‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y1480-p - tensin 1 (mouse)
Modsite: RHAAyGGystPEDRR SwissProt Entrez-Gene
Orthologous residues
tensin 1 (human): Y1326‑p, tensin 1 iso2 (human): , tensin 1 (mouse): Y1480‑p, tensin 1 (rat): Y1499‑p, tensin 1 (cow): Y1305‑p