Curated Information
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Home > Curated Information Page > Cst Curation Set #50
Guo A (2003) CST Curation Set: 50; Year: 2003; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y208-p - ARF GAP1 (human)
Modsite: NNAMSsLysGWSSFT SwissProt Entrez-Gene
Orthologous residues
ARF GAP1 (human): Y208‑p, ARF GAP1 iso2 (human): Y208‑p, ARF GAP1 (mouse): Y208‑p, ARF GAP1 (rat): Y208‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y75-p - DFFA (human)
Modsite: tIVDDDDyFLCLPSN SwissProt Entrez-Gene
Orthologous residues
DFFA (human): Y75‑p, DFFA iso2 (human): Y75‑p, DFFA (mouse): Y75‑p, DFFA iso2 (mouse): Y75‑p, DFFA (rat): Y75‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y75-p - DFFA iso2 (human)
Modsite: TIVDDDDyFLCLPSN SwissProt Entrez-Gene
Orthologous residues
DFFA (human): Y75‑p, DFFA iso2 (human): Y75‑p, DFFA (mouse): Y75‑p, DFFA iso2 (mouse): Y75‑p, DFFA (rat): Y75‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y251-p - EIF2A (human)
Modsite: DKTGAsyyGEQTLHy SwissProt Entrez-Gene
Orthologous residues
EIF2A (human): Y251‑p, EIF2A iso2 (human): Y37‑p, EIF2A (mouse): Y251‑p, EIF2A (rat): Y211‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y593-p - EIF4B (human)
Modsite: kFSsAskyAALsVDG SwissProt Entrez-Gene
Orthologous residues
EIF4B (human): Y593‑p, EIF4B (mouse): Y593‑p, EIF4B (rat): Y593‑p, EIF4B (cow): Y593‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y609-p - EIF4B (human)
Modsite: DENEGEDyAE_____ SwissProt Entrez-Gene
Orthologous residues
EIF4B (human): Y609‑p, EIF4B (mouse): C609‑p, EIF4B (rat): C609‑p, EIF4B (cow): Y608‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y545-p - GIT1 (human)
Modsite: ELEDDAIysVHVPAG SwissProt Entrez-Gene
Orthologous residues
GIT1 (human): Y545‑p, GIT1 iso3 (human): Y554‑p, GIT1 (mouse): Y554‑p, GIT1 (rat): Y554‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y505-p - Lck (human)
Modsite: FTAtEGQyQPQP___ SwissProt Entrez-Gene
Orthologous residues
Lck (human): Y505‑p, Lck iso3 (human): Y535‑p, Lck (mouse): Y505‑p, Lck (rat): Y505‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y272-p - MPST (human)
Modsite: GKPDVPIyDGSWVEW SwissProt Entrez-Gene
Orthologous residues
MPST (human): Y272‑p, MPST iso2 (human): Y292‑p, MPST iso3 (human): Y292‑p, MPST (mouse): Y272‑p, MPST (rat): Y272‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y780-p - SLAP-130 (human)
Modsite: DIAdGCIyDND____ SwissProt Entrez-Gene
Orthologous residues
SLAP‑130 (human): Y780‑p, SLAP‑130 iso2 (human): Y826‑p, SLAP‑130 iso3 (human): Y836‑p, SLAP‑130 (mouse): Y816‑p, SLAP‑130 (rat): Y817‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y20-p - TfR (human)
Modsite: FGGEPLsytRFsLAR SwissProt Entrez-Gene
Orthologous residues
TfR (human): Y20‑p, TfR (mouse): Y20‑p, TfR (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y451-p - TUBA1B (human)
Modsite: GEEEGEEy_______ SwissProt Entrez-Gene
Orthologous residues
TUBA1B (human): Y451‑p, TUBA1B (mouse): Y451‑p, TUBA1B (rat): Y451‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y844-p - VAV1 (human)
Modsite: VEEDysEyC______ SwissProt Entrez-Gene
Orthologous residues
VAV1 (human): Y844‑p, VAV1 iso2 (human): Y812‑p, VAV1 (mouse): Y844‑p, VAV1 (rat): Y842‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human