Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.8
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 17394530
Nunbhakdi-Craig V, et al. (2007) Expression of protein phosphatase 2A mutants and silencing of the regulatory B alpha subunit induce a selective loss of acetylated and detyrosinated microtubules. J Neurochem 101, 959-71 17394530
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T304-p - PPP2CA (human)
Modsite: EPHVTRRtPDyFl__ SwissProt Entrez-Gene
Orthologous residues
PPP2CA (human): T304‑p, PPP2CA iso2 (human): T250‑p, PPP2CA (mouse): T304‑p, PPP2CA (rat): T304‑p, PPP2CA (pig): T304‑p, PPP2CA (cow): T304‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), Neuro-2a (neuron)
Cellular systems studied:  cell lines
Species studied:  mouse
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PPP2R3A (mouse) Induces co-immunoprecipitation
PPP2R5A (mouse) Disrupts co-immunoprecipitation
PPP2R5D (mouse) Induces co-immunoprecipitation
PPP2R2A (mouse) Not reported co-immunoprecipitation
Comments:  Done with T304A and T304D mutations as compared to WT. Not reported means both mutation effected binding in the same way.

Y307-p - PPP2CA (human)
Modsite: VTRRtPDyFl_____ SwissProt Entrez-Gene
Orthologous residues
PPP2CA (human): Y307‑p, PPP2CA iso2 (human): Y253‑p, PPP2CA (mouse): Y307‑p, PPP2CA (rat): Y307‑p, PPP2CA (pig): Y307‑p, PPP2CA (cow): Y307‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), Neuro-2a (neuron)
Cellular systems studied:  cell lines
Species studied:  mouse
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PPP2R5A (mouse) Not reported co-immunoprecipitation
PPP2R2A (mouse) Disrupts co-immunoprecipitation
PPP2R5D (mouse) Not reported co-immunoprecipitation
PPP2R3A (mouse) Disrupts co-immunoprecipitation
Comments:  Done with Y307F and Y307E mutations as compared to WT. Not reported means both mutation effected binding in the same way.