Curated Information
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Home > Curated Information Page > PubMed Id: 19223464
Bechard M, Dalton S (2009) Subcellular localization of glycogen synthase kinase 3beta controls embryonic stem cell self-renewal. Mol Cell Biol 29, 2092-104 19223464
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  ESC
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LIF increase decrease following LIF withdrawal
4-HT increase

S9-p - GSK3B (mouse)
Modsite: SGRPRttsFAEsCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  ESC
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LIF increase decrease following LIF withdrawal
4-HT increase
LY294002 decrease
BIO LY294002 no effect upon treatment-induced decrease

T58-p - Myc (mouse)
Modsite: KKFELLPtPPLsPsR SwissProt Entrez-Gene
Orthologous residues
Myc (human): T58‑p, Myc iso2 (human): T73‑p, Myc (mouse): T58‑p, Myc (rat): T58‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  ESC
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (mouse) inhibition of upstream enzyme, microscopy-colocalization
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LY294002 increase
BIO LY294002 inhibit treatment-induced increase
GSK3B (mouse), GSK3A (mouse) no change compared to control GSK3AB DKO
LIF decrease increase following LIF withdrawal
4-HT decrease
Downstream Regulation
Effect of modification (function):  protein degradation
Effect of modification (process):  cell differentiation, altered