Curated Information
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Home > Curated Information Page > Cst Curation Set #6758
Possemato A (2009) CST Curation Set: 6758; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y55-p - ACTA1 (human)
Modsite: GMGQkDsyVGDEAQs SwissProt Entrez-Gene
Orthologous residues
ACTA1 (human): Y55‑p, ACTA1 (mouse): Y55‑p, ACTA1 (rat): Y55‑p, ACTA1 (rabbit): Y55‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y55-p - ACTA2 (human)
Modsite: GMGQkDsyVGDEAQs SwissProt Entrez-Gene
Orthologous residues
ACTA2 (human): Y55‑p, ACTA2 (mouse): Y55‑p, ACTA2 (rat): Y55‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y53-p - ACTB (human)
Modsite: GMGQkDsyVGDEAQs SwissProt Entrez-Gene
Orthologous residues
ACTB (human): Y53‑p, ACTB (mouse): Y53‑p, ACTB (rat): Y53‑p, ACTB (cow): Y53‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y55-p - ACTC1 (human)
Modsite: GMGQkDsyVGDEAQs SwissProt Entrez-Gene
Orthologous residues
ACTC1 (human): Y55‑p, ACTC1 (mouse): Y55‑p, ACTC1 (rat): Y55‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y53-p - ACTG1 (human)
Modsite: GMGQkDsyVGDEAQs SwissProt Entrez-Gene
Orthologous residues
ACTG1 (human): Y53‑p, ACTG1 (mouse): Y53‑p, ACTG1 (rat): Y53‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y54-p - ACTG2 (human)
Modsite: GMGQkDsyVGDEAQs SwissProt Entrez-Gene
Orthologous residues
ACTG2 (human): Y54‑p, ACTG2 (mouse): Y54‑p, ACTG2 (rat): Y54‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y922-p - ANKS1A (human)
Modsite: PPSLAAPyAPVQSWQ SwissProt Entrez-Gene
Orthologous residues
ANKS1A (human): Y922‑p, ANKS1A (mouse): Y938‑p, ANKS1A (rat): Y913‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y172-p - DNAJB4 (human)
Modsite: RVsLEEIySGCTkRM SwissProt Entrez-Gene
Orthologous residues
DNAJB4 (human): Y172‑p, DNAJB4 (mouse): Y172‑p, DNAJB4 (rat): Y172‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y187-p - ERK2 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y240-p - hnRNP A/B (human)
Modsite: EVyQQQQyGsGGrGN SwissProt Entrez-Gene
Orthologous residues
hnRNP A/B (human): Y240‑p, hnRNP A/B iso2 (human): Y240‑p, hnRNP A/B iso3 (human): Y240‑p, hnRNP A/B (mouse): Y245‑p, hnRNP A/B iso2 (mouse): Y238‑p, hnRNP A/B (rat): Y244‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y176-p - HSP40 (human)
Modsite: RVsLEEIysGCtkKM SwissProt Entrez-Gene
Orthologous residues
HSP40 (human): Y176‑p, HSP40 (mouse): Y176‑p, HSP40 (rat): Y176‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T183-p - JNK1 (human)
Modsite: AGtsFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y185-p - JNK1 (human)
Modsite: tsFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T221-p - JNK3 (human)
Modsite: AGtsFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK3 (human): T221‑p, JNK3 iso2 (human): T221‑p, JNK3 (mouse): T221‑p, JNK3 iso2 (mouse): T221‑p, JNK3 iso3 (mouse): , JNK3 (rat): T221‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y223-p - JNK3 (human)
Modsite: tsFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK3 (human): Y223‑p, JNK3 iso2 (human): Y223‑p, JNK3 (mouse): Y223‑p, JNK3 iso2 (mouse): Y223‑p, JNK3 iso3 (mouse): , JNK3 (rat): Y223‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

S19-p - leupaxin (human)
Modsite: ERstLQDsDEysNPA SwissProt Entrez-Gene
Orthologous residues
leupaxin (human): S19‑p, leupaxin (mouse): S19‑p, leupaxin (rat): S19‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y22-p - leupaxin (human)
Modsite: tLQDsDEysNPAPLP SwissProt Entrez-Gene
Orthologous residues
leupaxin (human): Y22‑p, leupaxin (mouse): Y22‑p, leupaxin (rat): Y22‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y359-p - PAG (human)
Modsite: PssCNDLyAtVKDFE SwissProt Entrez-Gene
Orthologous residues
PAG (human): Y359‑p, PAG (mouse): Y356‑p, PAG (rat): Y351‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

S304-p - PKCD (human)
Modsite: RAsRRsDsAssEPVG SwissProt Entrez-Gene
Orthologous residues
PKCD (human): S304‑p, PKCD iso2 (human): S304‑p, PKCD (mouse): , PKCD iso2 (mouse): , PKCD (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y313-p - PKCD (human)
Modsite: ssEPVGIyQGFEKkT SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y313‑p, PKCD iso2 (human): Y313‑p, PKCD (mouse): Y311‑p, PKCD iso2 (mouse): Y311‑p, PKCD (rat): Y311‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y753-p - PLCG2 (human)
Modsite: ERDINSLyDVsRMyV SwissProt Entrez-Gene
Orthologous residues
PLCG2 (human): Y753‑p, PLCG2 (mouse): Y753‑p, PLCG2 (rat): Y753‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y164-p - ZAP70 (human)
Modsite: AHERMPWyHssLTRE SwissProt Entrez-Gene
Orthologous residues
ZAP70 (human): Y164‑p, ZAP70 (mouse): Y164‑p, ZAP70 (rat): Y164‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human