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Home > Curated Information Page > PubMed Id: 17244605
Shi Y, et al. (2007) Identification of carboxyl-terminal MCM3 phosphorylation sites using polyreactive phosphospecific antibodies. J Biol Chem 282, 9236-43 17244605
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S728-p - MCM3 (human)
Modsite: HtPKtADsQETkEsQ SwissProt Entrez-Gene
Orthologous residues
MCM3 (human): S728‑p, MCM3 iso2 (human): S773‑p, MCM3 (mouse): S732‑p, MCM3 (rat): S734‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATR (mouse) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
KINASE ATM (mouse) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
KU-55933 UV inhibit treatment-induced increase
siRNA UV inhibit treatment-induced increase ATM siRNA
KU-55933, siRNA UV inhibit treatment-induced increase ATM siRNA

S1987-p - ATM (mouse)
Modsite: SPTFEEGsQGTTIsS SwissProt Entrez-Gene
Orthologous residues
ATM (human): S1981‑p, ATM (mouse): S1987‑p, ATM (rat): S1988‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
KU-55933 inhibit treatment-induced increase
siRNA UV inhibit treatment-induced increase ATR siRNA
KU-55933, siRNA UV augment treatment-induced decrease
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  Preferentially localized to the soluble nucleoplasmic fraction.

S107-p - CREB (mouse)
Modsite: sVDsVTDsQKRREIL SwissProt Entrez-Gene
Orthologous residues
CREB (human): S107‑p, CREB iso1 (human): S121‑p, CREB (mouse): S107‑p, CREB iso1 (mouse): S121‑p, CREB (rat): S121‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATR (mouse) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
KINASE ATM (mouse) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
KU-55933 inhibit treatment-induced increase
siRNA UV inhibit treatment-induced increase ATR siRNA
KU-55933, siRNA UV augment treatment-induced decrease
hydroxyurea increase ATM +/+ MEFs
hydroxyurea inhibit treatment-induced increase ATM -/- MEFs
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  Preferentially localized to the soluble nucleoplasmic fraction.

S725-p - MCM3 (mouse)
Modsite: HtPKtDDsQEKtDDs SwissProt Entrez-Gene
Orthologous residues
MCM3 (human): , MCM3 iso2 (human): , MCM3 (mouse): S725‑p, MCM3 (rat): S727‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), MEF (fibroblast) [ATM (mouse)]
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ATM (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (mouse) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
KINASE ATR (mouse) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
KU-55933 inhibit treatment-induced increase
siRNA UV inhibit treatment-induced increase ATR siRNA
KU-55933, siRNA UV augment treatment-induced decrease
hydroxyurea increase ATM +/+ MEFs
hydroxyurea inhibit treatment-induced increase ATM -/- MEFs
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  Preferentially localized to the soluble nucleoplasmic fraction.

S732-p - MCM3 (mouse)
Modsite: sQEKtDDsQEtQDsQ SwissProt Entrez-Gene
Orthologous residues
MCM3 (human): S728‑p, MCM3 iso2 (human): S773‑p, MCM3 (mouse): S732‑p, MCM3 (rat): S734‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), MEF (fibroblast) [ATM (mouse)]
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ATM (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (mouse) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
KINASE ATR (mouse) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
KU-55933 inhibit treatment-induced increase
siRNA UV inhibit treatment-induced increase ATR siRNA
KU-55933, siRNA UV augment treatment-induced decrease
hydroxyurea increase ATM +/+ MEFs
hydroxyurea inhibit treatment-induced increase ATM -/- MEFs
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  Preferentially localized to the soluble nucleoplasmic fraction.