Curated Information
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Home > Curated Information Page > PubMed Id: 12149249
Conus NM, et al. (2002) Direct identification of tyrosine 474 as a regulatory phosphorylation site for the Akt protein kinase. J Biol Chem 277, 38021-8 12149249
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S124-p - Akt1 (human)
Modsite: EMDFRsGsPsDNsGA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S124‑p, Akt1 iso2 (human): S62‑p, Akt1 (mouse): S124‑p, Akt1 (rat): S124‑p, Akt1 (fruit fly): T236‑p, Akt1 (cow): S124‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody, phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), SKOV-3 (ovarian)
Cellular systems studied:  cell lines
Species studied:  green monkey, human

S129-p - Akt1 (human)
Modsite: sGsPsDNsGAEEMEV SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S129‑p, Akt1 iso2 (human): S67‑p, Akt1 (mouse): S129‑p, Akt1 (rat): S129‑p, Akt1 (fruit fly): E241‑p, Akt1 (cow): S129‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody, phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), SKOV-3 (ovarian)
Cellular systems studied:  cell lines
Species studied:  green monkey, human

T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), SKOV-3 (ovarian)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase
IGF-1 increase

T450-p - Akt1 (human)
Modsite: tAQMItItPPDQDDs SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T450‑p, Akt1 iso2 (human): T388‑p, Akt1 (mouse): T450‑p, Akt1 (rat): T450‑p, Akt1 (fruit fly): T565‑p, Akt1 (cow): T450‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody, phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), SKOV-3 (ovarian)
Cellular systems studied:  cell lines
Species studied:  green monkey, human

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), SKOV-3 (ovarian)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase
IGF-1 increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

Y474-p - Akt1 (human)
Modsite: PHFPQFsysAsGtA_ SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): Y474‑p, Akt1 iso2 (human): Y412‑p, Akt1 (mouse): Y474‑p, Akt1 (rat): Y474‑p, Akt1 (fruit fly): Y587‑p, Akt1 (cow): Y474‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), SKOV-3 (ovarian)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase
IGF-1 increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced