Curated Information
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Home > Curated Information Page > PubMed Id: 15004009
Mayer C, Zhao J, Yuan X, Grummt I (2004) mTOR-dependent activation of the transcription factor TIF-IA links rRNA synthesis to nutrient availability. Genes Dev 18, 423-34 15004009
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S44-p - TIF1A (human)
Modsite: LENDFFNsPPRKTVR SwissProt Entrez-Gene
Orthologous residues
TIF1A (human): S44‑p, TIF1A (mouse): S42‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), HEK293T (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)
KINASE CDK2 (human)
Comments:  Cdk2/Cyclin E
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rapamycin decrease
calyculin_A rapamycin inhibit treatment-induced decrease
Downstream Regulation
Effect of modification (function):  activity, induced
Effect of modification (process):  transcription, altered

S199-p - TIF1A (human)
Modsite: IIARYVPstPWFLMP SwissProt Entrez-Gene
Orthologous residues
TIF1A (human): S199‑p, TIF1A (mouse): S197‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), HEK293T (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rapamycin increase
Downstream Regulation
Effect of modification (function):  activity, inhibited, molecular association, regulation
Effect of modification (process):  transcription, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
TAF4 (human) Disrupts transcription, altered co-immunoprecipitation
eIF3S6IP (human) Disrupts co-immunoprecipitation