Curated Information
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Home > Curated Information Page > PubMed Id: 14741046
Sabio G, et al. (2004) Stress- and mitogen-induced phosphorylation of the synapse-associated protein SAP90/PSD-95 by activation of SAPK3/p38gamma and ERK1/ERK2. Biochem J 380, 19-30 14741046
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T203-p - ERK1 (rat)
Modsite: HDHTGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
SB203580 sorbitol no effect upon treatment-induced increase
PD184352 sorbitol inhibit treatment-induced increase
PD184352, SB203580 sorbitol inhibit treatment-induced increase
UV increase
SB203580 UV inhibit treatment-induced increase
PD184352 UV inhibit treatment-induced increase
PD184352, SB203580 UV inhibit treatment-induced increase

Y205-p - ERK1 (rat)
Modsite: HTGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
SB203580 sorbitol no effect upon treatment-induced increase
PD184352 sorbitol inhibit treatment-induced increase
PD184352, SB203580 sorbitol inhibit treatment-induced increase
UV increase
SB203580 UV inhibit treatment-induced increase
PD184352 UV inhibit treatment-induced increase
PD184352, SB203580 UV inhibit treatment-induced increase

T183-p - ERK2 (rat)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
SB203580 sorbitol no effect upon treatment-induced increase
PD184352 sorbitol inhibit treatment-induced increase
PD184352, SB203580 sorbitol inhibit treatment-induced increase
UV increase
SB203580 UV inhibit treatment-induced increase
PD184352 UV inhibit treatment-induced increase
PD184352, SB203580 UV inhibit treatment-induced increase

Y185-p - ERK2 (rat)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
SB203580 sorbitol no effect upon treatment-induced increase
PD184352 sorbitol inhibit treatment-induced increase
PD184352, SB203580 sorbitol inhibit treatment-induced increase
UV increase
SB203580 UV inhibit treatment-induced increase
PD184352 UV inhibit treatment-induced increase
PD184352, SB203580 UV inhibit treatment-induced increase

S15-p - HSP27 (rat)
Modsite: FsLLRsPsWEPFRDW SwissProt Entrez-Gene
Orthologous residues
HSP27 (human): S15‑p, HSP27 (mouse): S15‑p, HSP27 (rat): S15‑p, HSP27 (pig): S15‑p, HSP27 (hamster): S15‑p, HSP27 (chicken): S15‑p, HSP27 (dog): S15‑p, HSP27 (cow): S15‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
SB203580 UV inhibit treatment-induced increase
PD184352 UV augment treatment-induced increase
PD184352, SB203580 UV augment treatment-induced decrease

T180-p - P38A (rat)
Modsite: RHTDDEMtGyVATRW SwissProt Entrez-Gene
Orthologous residues
P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p, P38A (salmonid): T181‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
SB203580 sorbitol augment treatment-induced increase
PD184352 sorbitol no effect upon treatment-induced increase
PD184352, SB203580 sorbitol augment treatment-induced increase
UV increase
SB203580 UV no effect upon treatment-induced increase
PD184352 UV no effect upon treatment-induced increase
PD184352, SB203580 UV no effect upon treatment-induced increase

Y182-p - P38A (rat)
Modsite: TDDEMtGyVATRWYR SwissProt Entrez-Gene
Orthologous residues
P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
SB203580 sorbitol augment treatment-induced increase
PD184352 sorbitol no effect upon treatment-induced increase
PD184352, SB203580 sorbitol augment treatment-induced increase
UV increase
SB203580 UV no effect upon treatment-induced increase
PD184352 UV no effect upon treatment-induced increase
PD184352, SB203580 UV no effect upon treatment-induced increase

T287-p - PSD-95 (rat)
Modsite: TDyPtAMtPTsPRRY SwissProt Entrez-Gene
Orthologous residues
PSD‑95 (human): T287‑p, PSD‑95 (mouse): T287‑p, PSD‑95 (rat): T287‑p
Characterization
Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), PC-12 (chromaffin)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE P38G (human)
KINASE P38D (human)
KINASE ERK2 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
PP2 sorbitol inhibit treatment-induced increase

S290-p - PSD-95 (rat)
Modsite: PtAMtPTsPRRYsPV SwissProt Entrez-Gene
Orthologous residues
PSD‑95 (human): S290‑p, PSD‑95 (mouse): S290‑p, PSD‑95 (rat): S290‑p
Characterization
Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), PC-12 (chromaffin)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
KINASE P38G (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
UV increase
SB203580 sorbitol augment treatment-induced increase
PD184352 sorbitol no effect upon treatment-induced increase
PD184352, SB203580 sorbitol no effect upon treatment-induced increase
SB203580 UV no effect upon treatment-induced increase
PD184352 UV inhibit treatment-induced increase
PD184352 UV no effect upon treatment-induced increase
peptide inhibitor sorbitol inhibit treatment-induced increase
peptide inhibitor UV inhibit treatment-induced increase
EGF increase
phorbol_ester increase
SB203580 EGF augment treatment-induced increase
PD184352, SB203580 EGF inhibit treatment-induced increase
PD184352 EGF inhibit treatment-induced increase
SB203580 phorbol_ester no effect upon treatment-induced increase
PD184352 phorbol_ester inhibit treatment-induced increase
PD184352, SB203580 phorbol_ester inhibit treatment-induced increase
peptide inhibitor EGF no effect upon treatment-induced increase
sorbitol increase
SB203580 sorbitol no effect upon treatment-induced increase
PD184352 no effect upon treatment-induced increase
UV increase
SB203580 UV no effect upon treatment-induced increase
PD184352 UV no effect upon treatment-induced increase
PD184352, SB203580 inhibit treatment-induced increase
EGF increase
SB203580 EGF augment treatment-induced increase
PD184352 EGF inhibit treatment-induced increase
PD184352, SB203580 EGF inhibit treatment-induced increase
phorbol_ester increase
PD184352 phorbol_ester inhibit treatment-induced increase
PD184352, SB203580 phorbol_ester inhibit treatment-induced increase
SB203580 phorbol_ester inhibit treatment-induced increase