Curated Information
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Home > Curated Information Page > PubMed Id: 10713673
Zrihan-Licht S, et al. (2000) RAFTK/Pyk2 tyrosine kinase mediates the association of p190 RhoGAP with RasGAP and is involved in breast cancer cell invasion. Oncogene 19, 1318-28 10713673
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y1091-p - ARHGAP5 (human)
Modsite: DMDPsDNyAEPIDTI SwissProt Entrez-Gene
Orthologous residues
ARHGAP5 (human): Y1091‑p, ARHGAP5 iso2 (human): Y1091‑p, ARHGAP5 (mouse): Y1091‑p, ARHGAP5 iso3 (mouse): Y1091‑p, ARHGAP5 (rat): Y1090‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human

Y1109-p - ARHGAP5 (human)
Modsite: kGysDEIyVVPDDsQ SwissProt Entrez-Gene
Orthologous residues
ARHGAP5 (human): Y1109‑p, ARHGAP5 iso2 (human): Y1109‑p, ARHGAP5 (mouse): Y1109‑p, ARHGAP5 iso3 (mouse): Y1109‑p, ARHGAP5 (rat): Y1108‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) co-immunoprecipitation, transfection of wild-type enzyme
KINASE Src (human) co-immunoprecipitation, transfection of wild-type enzyme

Y402-p - Pyk2 (human)
Modsite: CsIEsDIyAEIPDEt SwissProt Entrez-Gene
Orthologous residues
Pyk2 (human): Y402‑p, Pyk2 iso2 (human): Y402‑p, Pyk2 (mouse): Y402‑p, Pyk2 (rat): Y402‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
heregulin increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell motility, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
RASA1 (human) Induces molecular association, regulation co-immunoprecipitation
HER2 (human) Induces molecular association, regulation co-immunoprecipitation
Src (human) Induces molecular association, regulation, enzymatic activity, induced co-immunoprecipitation
Associated Diseases
Diseases Alterations Comments
breast cancer increased breast carcinoma cell invasion