Suelves M, et al. (2004) Phosphorylation of MRF4 transactivation domain by p38 mediates repression of specific myogenic genes. EMBO J 23, 365-75 14739931

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

S31-p - Myf-6 (mouse) ▲

Modsite: PLEVAEGsPLYPGSD SwissProt Entrez-Gene Orthologous residues Myf‑6 (human): S31‑p, Myf‑6 (mouse): S31‑p, Myf‑6 (rat): S31‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), C2C12 (myoblast), C3H10T1/2 (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE P38A (mouse) modification site within consensus motif, co-immunoprecipitation, activation of upstream enzyme, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes MKK6 (mouse) increase SB203580 MKK6 (mouse) inhibit treatment-induced increase serum_withdrawal increase differentiation media (switch from 10% FBS to 2% HS) Downstream Regulation Effect of modification (process):  cell differentiation, altered, transcription, inhibited

S42-p - Myf-6 (mouse) ▲

Modsite: PGSDGTLsPCQDQMP SwissProt Entrez-Gene Orthologous residues Myf‑6 (human): S42‑p, Myf‑6 (mouse): S42‑p, Myf‑6 (rat): S42‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), C2C12 (myoblast), C3H10T1/2 (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE P38A (mouse) modification site within consensus motif, co-immunoprecipitation, activation of upstream enzyme, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes MKK6 (mouse) increase SB203580 MKK6 (mouse) inhibit treatment-induced increase serum_withdrawal increase differentiation media (switch from 10% FBS to 2% HS) Downstream Regulation Effect of modification (process):  cell differentiation, altered, transcription, inhibited