Haycock JW, Haycock DA (1991) Tyrosine hydroxylase in rat brain dopaminergic nerve terminals. Multiple-site phosphorylation in vivo and in synaptosomes. J Biol Chem 266, 5650-7 1672315

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S8-p - TH (rat) ▲

Modsite: MPTPSAPsPQPKGFR SwissProt Entrez-Gene Orthologous residues TH (human): T8‑p, TH iso2 (human): T8‑p, TH iso3 (human): T8‑p, TH iso4 (human): T8‑p, TH (mouse): S8‑p, TH (rat): S8‑p, TH (cow): S8‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping Cellular systems studied:  tissue Species studied:  rat Comments:  These studies were performed by perfusing P32 into whole rat brain corpus striata or P32-labelling of rat corpus striatal synaptosomes. Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester no change compared to control electrical_stimulation no change compared to control colforsin no change compared to control depolarization no change compared to control

S19-p - TH (rat) ▲

Modsite: KGFRRAVsEQDAKQA SwissProt Entrez-Gene Orthologous residues TH (human): S19‑p, TH iso2 (human): S19‑p, TH iso3 (human): S19‑p, TH iso4 (human): S19‑p, TH (mouse): S19‑p, TH (rat): S19‑p, TH (cow): S19‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping Cellular systems studied:  tissue Species studied:  rat Comments:  These studies were performed by perfusing P32 into whole rat brain corpus striata or P32-labelling of rat corpus striatal synaptosomes. Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes depolarization increase electrical_stimulation increase phorbol_ester no change compared to control colforsin no change compared to control

S31-p - TH (rat) ▲

Modsite: KQAEAVtsPRFIGRR SwissProt Entrez-Gene Orthologous residues TH (human): S62‑p, TH iso2 (human): S58‑p, TH iso3 (human): S31‑p, TH iso4 (human): S35‑p, TH (mouse): S31‑p, TH (rat): S31‑p, TH (cow): S31‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping Cellular systems studied:  tissue Species studied:  rat Comments:  These studies were performed by perfusing P32 into whole rat brain corpus striata or P32-labelling of rat corpus striatal synaptosomes. Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKCB (rat) pharmacological activator of upstream enzyme KINASE PKCB (rat) pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase electrical_stimulation increase colforsin no change compared to control depolarization no change compared to control

S40-p - TH (rat) ▲

Modsite: RFIGRRQsLIEDARK SwissProt Entrez-Gene Orthologous residues TH (human): S71‑p, TH iso2 (human): S67‑p, TH iso3 (human): S40‑p, TH iso4 (human): S44‑p, TH (mouse): S40‑p, TH (rat): S40‑p, TH (cow): S40‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping Cellular systems studied:  tissue Species studied:  rat Comments:  These studies were performed by perfusing P32 into whole rat brain corpus striata or P32-labelling of rat corpus striatal synaptosomes. Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKACA (rat) pharmacological activator of upstream enzyme KINASE PKACA (rat) pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes colforsin increase electrical_stimulation increase phorbol_ester no change compared to control depolarization no change compared to control