Curated Information
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Home > Curated Information Page > PubMed Id: 16935857
Kramer HF, et al. (2006) AS160 regulates insulin- and contraction-stimulated glucose uptake in mouse skeletal muscle. J Biol Chem 281, 31478-85 16935857
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T308-p - Akt1 (mouse)
Modsite: KDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  mouse

T172-p - AMPKA2 (mouse)
Modsite: sDGEFLRtsCGsPNY SwissProt Entrez-Gene
Orthologous residues
AMPKA2 (human): T172‑p, AMPKA2 (mouse): T172‑p, AMPKA2 (rat): T172‑p, AMPKA2 (pig): T172‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  mouse

S324-p - AS160 (mouse)
Modsite: EFRsRCssVTGVMQK SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S318‑p, AS160 iso2 (human): S318‑p, AS160 iso3 (human): S318‑p, AS160 (mouse): S324‑p, AS160 iso2 (mouse): S324‑p, AS160 (rat): S326‑p, AS160 (cow): S319‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S595-p - AS160 (mouse)
Modsite: RMRGRLGsMDsFERA SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S588‑p, AS160 iso2 (human): S588‑p, AS160 iso3 (human): S588‑p, AS160 (mouse): S595‑p, AS160 iso2 (mouse): S595‑p, AS160 (rat): S597‑p, AS160 (cow): S590‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T649-p - AS160 (mouse)
Modsite: QFRRRAHtFsHPPSS SwissProt Entrez-Gene
Orthologous residues
AS160 (human): T642‑p, AS160 iso2 (human): T642‑p, AS160 iso3 (human): T642‑p, AS160 (mouse): T649‑p, AS160 iso2 (mouse): T649‑p, AS160 (rat): T651‑p, AS160 (cow): T644‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 3T3-L1 (fibroblast)
Cellular systems studied:  cell lines, tissue
Species studied:  mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (mouse) phospho-motif antibody
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
muscle contraction increase

S758-p - AS160 (mouse)
Modsite: EGRKRtsstCsNEsL SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S751‑p, AS160 iso2 (human): S688‑p, AS160 iso3 (human): S743‑p, AS160 (mouse): S758‑p, AS160 iso2 (mouse): S695‑p, AS160 (rat): S760‑p, AS160 (cow): S753‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse